Neural Regeneration Research ›› 2017, Vol. 12 ›› Issue (6): 918-924.doi: 10.4103/1673-5374.208573

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Neuroprotective effects of ischemic preconditioning on hippocampal CA1 pyramidal neurons through maintaining calbindin D28k immunoreactivity following subsequent transient cerebral ischemia

 In Hye Kim1, Yong Hwan Jeon2, Tae-Kyeong Lee1, Jeong Hwi Cho1, Jae-Chul Lee1, Joon Ha Park3, Ji Hyeon Ahn3, Bich-Na Shin4, Yang Hee Kim5, Seongkweon Hong5, Bing Chun Yan6, Moo-Ho Won1, Yun Lyul Lee4   

  1. 1 Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea; 2 Department of Radiology, School of Medicine, Kangwon National University, Chuncheon, South Korea; 3 Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, South Korea; 4 Department of Physiology, College of Medicine, Hallym University, Chuncheon, South Korea; 5 Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, South Korea; 6 Institute of Integrative Traditional & Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu Province, China
  • Received:2017-05-15 Online:2017-06-15 Published:2017-06-15
  • Contact: Moo-Ho Won, D.V.M., Ph.D., or Yun Lyul Lee, D.V.M., Ph.D., mhwon@kangwon.ac.kr or yylee@hallym.ac.kr.
  • Supported by:

    This research was supported by Hallym University Research Fund, 2016 (HRF-201605-012), and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1A6A3A01011698).

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Abstract:

Ischemic preconditioning elicited by a non-fatal brief occlusion of blood flow has been applied for an experimental therapeutic strategy against a subsequent fatal ischemic insult. In this study, we investigated the neuroprotective effects of ischemic preconditioning (2-minute transient cerebral ischemia) on calbindin D28k immunoreactivity in the gerbil hippocampal CA1 area following a subsequent fatal transient ischemic insult (5-minute transient cerebral ischemia). A large number of pyramidal neurons in the hippocampal CA1 area died 4 days after 5-minute transient cerebral ischemia. Ischemic preconditioning reduced the death of pyramidal neurons in the hippocampal CA1 area. Calbindin D28k immunoreactivity was greatly attenuated at 2 days after 5-minute transient cerebral ischemia and it was hardly detected at 5 days post-ischemia. Ischemic preconditioning maintained calbindin D28k immunoreactivity after transient cerebral ischemia. These findings suggest that ischemic preconditioning can attenuate transient cerebral ischemia-caused damage to the pyramidal neurons in the hippocampal CA1 area through maintaining calbindin D28k immunoreactivity.

Key words: nerve regeneration, transient cerebral ischemia, ischemic tolerance, neuroprotection, hippocampus, pyramidal neurons, calcium binding protein, neural regeneration