Neural Regeneration Research ›› 2019, Vol. 14 ›› Issue (3): 525-531.doi: 10.4103/1673-5374.245478

Previous Articles     Next Articles

Increased levels of miR-3099 induced by peripheral nerve injury promote Schwann cell proliferation and migration

Qian-Yan Liu 1, Yang Miao 2, Xing-Hui Wang 1 , Pan Wang 1 , Zhang-Chun Cheng 1 , Tian-Mei Qian 1   

  1. 1 Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
    2 Department of Pharmacy, Yancheng City No. 1 People’s Hospital, Yancheng, Jiangsu Province, China
  • Online:2019-03-15 Published:2019-03-15
  • Contact: Tian-Mei Qian, qiantm86@ntu.edu.cn.
  • Supported by:

    This study was supported by the Postgraduate Research & Practice Innovation Program of Jiangsu Province of China, No. KYCX17-1910 (to QYL); and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions of China ( PAPD).

PDF

212

Abstract:

MicroRNAs (miRNAs) can regulate the modulation of the phenotype of Schwann cells. Numerous novel miRNAs have been discovered and identified in rat sciatic nerve segments, including miR-3099. In the current study, miR-3099 expression levels following peripheral nerve injury were measured in the proximal stumps of rat sciatic nerves after surgical crush. Real-time reverse transcription-polymerase chain reaction was used to determine miR-3099 expression in the crushed nerve segment at 0, 1, 4, 7, and 14 days post sciatic nerve injury, which was consistent with Solexa sequencing outcomes. Expression of miR-3099 was up-regulated following peripheral nerve injury. EdU and tran¬swell chamber assays were used to observe the effect of miR-3099 on Schwann cell proliferation and migration. The results showed that increased miR-3099 expression promoted the proliferation and migration of Schwann cells. However, reduced miR-3099 expression sup¬pressed the proliferation and migration of Schwann cells. The potential target genes of miR-3099 were also investigated by bioinformatic tools and high-throughput outcomes. miR-3099 targets genes Aqp4, St8sia2, Tnfsf15, and Zbtb16 and affects the proliferation and mi¬gration of Schwann cells. This study examined the levels of miR-3099 at different time points following peripheral nerve injury. Our results confirmed that increased miR-3099 level induced by peripheral nerve injury can promote the proliferation and migration of Schwann cells.

Key words: nerve regeneration, peripheral nerve injury, miR-3099, sciatic nerve, gene expression, Schwann cells, proliferation, migration, target genes, mechanisms, neural regeneration