Neural Regeneration Research ›› 2020, Vol. 15 ›› Issue (1): 86-95.doi: 10.4103/1673-5374.264469

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Single-nucleotide polymorphism screening and RNA sequencing of key messenger RNAs associated with neonatal hypoxic-ischemia brain damage

Liu-Lin Xiong1, 2, Lu-Lu Xue3, Mohammed Al-Hawwas2, Jin Huang3, Rui-Ze Niu3, Ya-Xin Tan3, Yang Xu4, Ying-Ying Su4, Jia Liu3,#br# Ting-Hua Wang3, 4#br#   

  1. 1 Department of Anesthesiology, National Traditional Chinese Medicine Clinical Research Base and Western Medicine Translational Medicine Research Center, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan Province, China

    2 School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, South Australia, Australia

    3 Department of Animal Zoology, Kunming Medical University, Kunming, Yunnan Province, China

    4 Institute of Neurological Disease, Department of Anesthesiology, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China

  • Online:2020-01-15 Published:2020-05-20
  • Contact: Jia Liu, PhD, liujiaaixuexi@126.com; Ting-Hua Wang, PhD, wangtinghua@vip.163.com.
  • Supported by:
    This study was supported by the program Innovative Research Team in Science and Technology in Yunnan Province of China (to THW); the National Natural Science Foundation of China, No. 81601074, Sichuan Provincial Scientific Foundation Grant of China, No. 2017SZ0145.

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Abstract: A single-nucleotide polymorphism (SNP) is an alteration in one nucleotide in a certain position within a genome. SNPs are associated with disease susceptibility. However, the influences of SNPs on the pathogenesis of neonatal hypoxic-ischemic brain damage remain elusive. Seven- day-old rats were used to establish a hypoxic ischemic encephalopathy model. SNPs and expression profiles of mRNAs were analyzed in hypoxic ischemic encephalopathy model rats using RNA sequencing. Genes exhibiting SNPs associated with hypoxic ischemic encephalopathy were identified and studied by gene ontology and pathway analysis to identify their possible involvement in the disease mechanism. We identified 89 up-regulated genes containing SNPs that were mainly located on chromosome 1 and 2. Gene ontology analysis indicated that the up-regulated genes containing SNPs are mainly involved in angiogenesis, wound healing and glutamatergic synapse and biological processing of calcium-activated chloride channels. Signaling pathway analysis indicated that the differentially expressed genes play a role in glutamatergic synapses, long-term depression and oxytocin signaling. Moreover, intersection analysis of high throughput screening following PubMed retrieval and RNA sequencing for SNPs showed that CSRNP1, DUSP5 and LRRC25 were most relevant to hypoxic ischemic encephalopathy. Significant up-regulation of genes was confirmed by quantitative real-time polymerase chain reaction analysis of oxygen-glucose-deprived human fetal cortical neurons. Our results indicate that CSRNP1, DUSP5 and LRRC25, containing SNPs, may be involved in the pathogenesis of hypoxic ischemic encephalopathy. These findings indicate a novel direction for further hypoxic ischemic encephalopathy research. This animal study was approved on February 5, 2017 by the Animal Care and Use Committee of Kunming Medical University, Yunnan Province, China (approval No. kmmu2019038). Cerebral tissue collection from a human fetus was approved on September 30, 2015 by the Ethics Committee of Kunming Medical University, China (approval No. 2015-9).

Key words: CSRNP1, DUSP5, gene ontology analysis, human fetal cortical neurons, LRRC25, mRNA, neonatal hypoxic ischemic encephalopathy, pathogenesis, signaling pathway analysis