Abstract: Small Aδ and C nerve fibers of the sensory and autonomic nervous systems constitute 70–90% of peripheral nerve fibers including corneal nerves (Muller et al., 2003). Corneal nerves originate from the ophthalmic branch of the trigeminal nerve and enter the cornea at the limbus radially from all directions toward the central cornea at the level of anterior and middle stroma. The subepithelial nerve plexus lies at the interface between the Bowman layer and anterior stroma. They then divide into smaller branches and turn 90° toward Bowman’s layer (Muller et al., 2003), travelling between Bowman’s layer and the basal epithelial layer and forming the sub-basal nerve plexus (Muller et al., 2003). Innervation of the cornea is comprised almost entirely of unmyelinated type C nerve fibers, with the fiber width ranging between 0.2–2.0 μm. Corneal nerves not only provide important sensory function but also maintain the functional integrity of the ocular surface by releasing trophic substances that promote corneal epithelial homeostasis and by activating brainstem circuits that stimulate reflex tear production and blinking (Marfurt et al., 2010). Disruption of corneal nerves result in reduced or absent corneal sensations as well as negative impacts on the ocular surface integrity and tear film dynamics, leading to dry eye symptoms, corneal epithelial breakdown, and neurotrophic keratopathy (Al-Aqaba et al., 2019).