Neural Regeneration Research ›› 2021, Vol. 16 ›› Issue (10): 2000-2001.doi: 10.4103/1673-5374.308084

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Intravitreal fluorogold tracing as a method to label retinal neurons and the retinal pigment epithelium

Francisco Javier Valiente-Soriano*, Fernando Lucas-Ruiz, Juan A. Miralles de Imperial-Ollero, Manuel Vidal-Sanz, Marta Agudo-Barriuso*#br#   

  1. Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia and Instituto Murciano de Investigación Biosanitaria-Virgen de la Arrixaca (IMIB-Arrixaca) Murcia, Spain
  • Online:2021-10-15 Published:2021-03-19
  • Contact: Marta Agudo-Barriuso, martabar@um.es; Francisco Javier Valiente Soriano, PhD,fjvaliente@um.es.
  • Supported by:
    This work was supported by the Spanish Ministry of Economy and Competitiveness, Instituto de Salud Carlos III, Fondo Europeo de Desarrollo Regional “Una manera de hacer Europa” (PI19/00071 to MAB, PID2019-106498GB-I00, RD16/0008/0026 and RD16/0008/0016 to MVS) and by the Fundación Séneca, Agencia de Ciencia y Tecnología Región de Murcia (19881/GERM/15, to MVS).

Abstract: Techniques to label the neuroretina and the retinal pigment epithelium (RPE) have been the topic of many studies for years. The reliability and reproducibility of these techniques are essential to investigate retinal alterations of ocular pathologies and possible treatments. In particular, the study of the integrity of the RPE is of great importance in pathologies such as retinitis pigmentosa or age-related macular degeneration (Gu et al., 2012; Fisher and Ferrington, 2018; Zhang et al., 2019). The most common approaches use antibodies to identify different cell populations. Recently, we have described a novel technique to study the integrity of the neuroretina and the RPE, as well as the functionality of the RPE in rats (Valiente-Soriano et al., 2020).