Abstract: Membrane disruption is a pathology in which the cellular phospholipid bilayer, which is the cell’s primary defense from the extracellular environment, is compromised. Some neurons that are impacted by membrane disruption experience cell death, and others demonstrate delayed disruption, providing an opportunity for therapeutic intervention. This pathology is visualized using infusions of cell impermeable tracers, such as dextran, calcein and propidium iodide followed by microscopic analysis (Geddes et al., 2003a, b; Singleton and Povlishock, 2004; Farkas et al., 2006; Whalen et al., 2008; Cullen et al., 2011; Lafrenaye et al., 2014; Levine et al., 2016; Hernandez et al., 2019; LaPlaca et al., 2019; Prado and LaPlaca, 2020), and therefore little is known about membrane disruption clinically. However, membrane disruption has been well documented to occur in various pre-clinical models of traumatic brain injury (TBI).