Neural Regeneration Research ›› 2022, Vol. 17 ›› Issue (8): 1748-1750.doi: 10.4103/1673-5374.332138

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Flow cytometry and stroke: from current methodology to future applications

Sylvie Amu#, Kyle Malone*, #   

  1. Cancer Research @UCC, University College Cork, Cork, Ireland (Amu S)
    Department of Pharmacology and Therapeutics, Western Gateway Building, University College Cork, Cork, Ireland (Malone K)
    School of Pharmacy, University College Cork, Cork, Ireland (Malone K)
  • Online:2022-08-15 Published:2022-01-22
  • Contact: Kyle Malone,kyle.malone@ucc.ie.
  • Supported by:
    This work was generously supported by a Government of Ireland Postgraduate Scholarship from the Irish Research Council (No. GOIPG/2017/431), the Interreg Atlantic Area Programme (No. EAPA_791/2018, 2019–21), and Dermot Costello Cancer Immunology Fellowship, Breakthrough Cancer Research.

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Abstract: Flow cytometry is a versatile technique for analyzing stroke-induced changes in the immune system. Unlike other methods of cell identification such as immunohistochemistry, the technique is rapid, highly sensitive, and capable of quantifying multiple markers in cell suspensions. Utilizing cell sorters, flow cytometers can also produce highly enriched populations of viable cells for functional studies. The aim of this perspective is to appraise current flow cytometry methods in the field of stroke, provide guidance on best practices, and outline some of the future applications of the technique in pre-clinical and clinical stroke research.