Neural Regeneration Research ›› 2023, Vol. 18 ›› Issue (1): 162-169.doi: 10.4103/1673-5374.344842

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Nerve growth factor-basic fibroblast growth factor poly-lactide co-glycolid sustained-release microspheres and the small gap sleeve bridging technique to repair peripheral nerve injury

Ming Li1, 2, 3, #, Ting-Min Xu1, 2, #, Dian-Ying Zhang1, #, Xiao-Meng Zhang1, Feng Rao2, 3, Si-Zheng Zhan1, Man Ma1, Chen Xiong1, Xiao-Feng Chen1, Yan-Hua Wang1, *, #   

  1. 1Department of Trauma and Orthopedics, Peking University People’s Hospital, Beijing, China; 2Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education, Beijing, China; 3Trauma Medicine Center, Peking University People’s Hospital; National Center for Trauma Medicine, Beijing, China
  • Online:2023-01-15 Published:2022-06-17
  • Contact: Yan-Hua Wang, PhD, wangyanhua04119@pkuph.edu.cn.
  • Supported by:
    This work was supported by the National Key Research and Development Program of China, No. 2016YFC1101603 (to DYZ); the National Natural Science Foundation of China, Nos. 31640045 (to YHW), 81901251 (to ML); the Natural Science Foundation of Beijing of China, No. 7204323 (to ML).

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Abstract: We previously prepared nerve growth factor poly-lactide co-glycolid sustained-release microspheres to treat rat sciatic nerve injury using the small gap sleeve technique. Multiple growth factors play a synergistic role in promoting the repair of peripheral nerve injury; as a result, in this study, we added basic fibroblast growth factors to the microspheres to further promote nerve regeneration. First, in an in vitro biomimetic microenvironment, we developed and used a drug screening biomimetic microfluidic chip to screen the optimal combination of nerve growth factor/basic fibroblast growth factor to promote the regeneration of Schwann cells. We found that 22.56 ng/mL nerve growth factor combined with 4.29 ng/mL basic fibroblast growth factor exhibited optimal effects on the proliferation of primary rat Schwann cells. The successfully prepared nerve growth factor-basic fibroblast growth factor-poly-lactide-co-glycolid sustained-release microspheres were used to treat rat sciatic nerve transection injury using the small gap sleeve bridge technique. Compared with epithelium sutures and small gap sleeve bridging alone, the small gap sleeve bridging technique combined with drug-free sustained-release microspheres has a stronger effect on rat sciatic nerve transfection injury repair at the structural and functional level. 

Key words: biomimetic microfluidic chip, growth factor, in vitro biomimetic microenvironment, nerve function, peripheral nerve injury, sciatic nerve, small gap sleeve bridging, sustained-release microspheres