Neural Regeneration Research ›› 2023, Vol. 18 ›› Issue (8): 1789-1794.doi: 10.4103/1673-5374.360247

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Splenectomy does not affect mouse behaviors

Jiao-Qiong Guan1, 2, #, Pei-Sen Zhang1, #, Wen-Chao Zhang3, Bing-Qian Zhang2, Hai-Tao Wu4, 5, *, Yue Lan1, 6, *, Ti-Fei Yuan2, 5, *   

  1. 1Department of Rehabilitation Medicine, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong Province, China; 2Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; 3College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China; 4Department of Neurobiology, Beijing Institute of Basic Medical Sciences, Beijing, China; 5Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China; 6Guangzhou Key Laboratory of Aging Frailty & Neurorehabilitation, Guangzhou, Guangdong Province, China
  • Online:2023-08-15 Published:2023-02-24
  • Contact: Ti-Fei Yuan, PhD, ytf0707@126.com; Yue Lan, PhD, bluemooning@163.com; Hai-Tao Wu, PhD, wuht@bmi.ac.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81772438, 81974357, and the Guangzhou Municipal Science and Technology Program, No. 202206010197 (all to YL).

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Abstract: The spleen is critical for immunity. It is the largest immune organ and immune center in the peripheral system. While the relationship between behavior and immunity has been demonstrated in physiology and diseases, the role of the spleen in behavior is not clear. To investigate the effects of the spleen on behaviors, we performed a refined splenectomy procedure on C57BL/6J mice and performed an open field test, circadian rhythm test, elevated plus maze, sucrose preference test, and Barnes maze test. Splenectomy did not induce changes in general locomotion, circadian rhythms, learning and memory, or depression/anxiety-related behaviors. To further investigate the effects of spleen on stress susceptibility, we established mouse models of depression through chronic unpredictable mild stress. The behavioral performances of mice subjected to splenectomy showed no differences from control animals. These findings suggest that splenectomy does not cause changes in baseline behavioral performance in mice. 

Key words: anxiety, chronic unpredictable mild stress, circadian rhythms, depression, immunity, learning, locomotion, memory, spleen, splenectomy