Neural Regeneration Research ›› 2025, Vol. 20 ›› Issue (on line): 1-18.doi: 10.4103/NRR.NRR-D-25-00621

   

Complex bioactive nanofibrous dura mater promotes the repair of traumatic brain injury

Siyu Chen1, Xiaopei Zhang1, 2, Qingxia Guo1, Yuying Yan1, Manfei Fu1, Yuanfei Wang3, *, Tong Wu1, 2, *   

  1. 1The Affiliated Hospital of Qingdao University, School of Basic Medicine, Qingdao Medical College, Qingdao University, Qingdao, Shandong Province, China; 2Shandong Key Laboratory of Medical and Health Textile Materials, College of Textile & Clothing, Qingdao University, Qingdao, Shandong Province,China; 3Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, Shandong Province, China
  • Online:2025-01-01 Published:2025-08-06
  • Contact: Yuanfei Wang, PhD, zhizunbao19@163.com; Tong Wu, PhD, twu@qdu.edu.cn.
  • Supported by:
    This research was supported by National Natural Science Foundation of China, No. 32171322 (to WT); Special Funds for Taishan Scholars Project of Shandong Province, No. tsqn202211125 (to WT); Natural Science Foundation of Shandong Province, No. ZR2024JQ026 (to WT); Young Elite Scientists Sponsorship Program by CAST, No. YESS20200097 (to WT); Qingdao Key Health Discipline Development Fund, No. 2025-2027 (to YW); Qingdao Clinical Research Center for Oral Diseases, No. 22-3-7-lczx-7-nsh (to YW); Shandong Provincial Key Medical and Health Discipline of Oral Medicine, No. 2025-2027 (to YW); and the “Advanced Biomaterials and Regenerative Medicine (ABRM)” Innovation Team supported by the Young-Talent Introduction and Cultivation Plan in the Universities of Shandong Province.

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Abstract: Dura closure following the surgery of traumatic brain injury is important to keep the structural integrity of brain and serve as the barrier to prevent infection and the leakage of cerebrospinal fluid. Though artificial dural maters can provide barrier capability, the performance for injured neural cells repairing and neuroprotection in the stage of secondary injury are unsatisfied. Herein, we designed and manufactured a multilayered nanofiber dural mater for the repair of traumatic brain injury. The results showed that the multilayered nanofiber dural mater was able to promote the survival and neurite extension of SH-SY5Y cells following the injuries of neurite transection, oxygen and glucose deprivation and oxidative stress, respectively. Minocycline hydrochloride and insulin growth factor-1 were separately incorporated into fibers to realize the differentially dual release for the purpose of immunomodulation at early stage and exert neuroprotection ability during the repair process. In addition, the multilayered nanofiber dural mater promoted the increase of M2 polarization for microglia cells and the secretion of anti-inflammatory cytokines, enhancing the survival of neural cells. The capabilities of the multilayered nanofiber dural mater for antibacterial properties, anti-adhesion, barrier performance, anti-leakage and biocompatibility have also been verified. Taken together, such multilayered nanofiber dural mater has shown promising potential to serve as the substitutes for dura and promotingneural recovery after traumatic brain injury.

Key words: anti-adhesion, antibacterial, anti-inflammatory, anti-leakage, barrier performance, biocompatibility, differential dual-release, multilayered nanofiber dura mater, neuroprotection, traumatic brain injury, nerve regeneration