Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (33): 2576-2582.

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Underlying mechanism of protection from hypoxic injury seen with n-butanol extract of Potentilla anserine L. in hippocampal neurons

Xiaojing Qin1, Lingzhi Li 2, 3, Qi Lv4, Baoguo Yu5, Shuwang Yang6, Tao He1, Yongliang Zhang 3, 7   

  1. 1 Department of Pathology, Affiliated Hospital of Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
    2 Department of Medicinal Chemistry, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
    3 Tianjin Key Laboratory of Occupational and Environmental Hazard Biomarkers, Tianjin 300162, China
    4 Department of Central Laboratory, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
    5 Department of Rescue Medicine, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
    6 Department of Postgraduate, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
    7 Ministry of Scientific Research, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
  • Received:2012-03-31 Revised:2012-07-26 Online:2012-11-25 Published:2012-11-25
  • Contact: Yongliang Zhang, M.D.,Professor, Tianjin Key Laboratory of Occupational and Environmental Hazard Biomarkers, Tianjin 300162,China; Ministry of Scientific Research, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162,China ; Lingzhi Li, M.D.,Professor, Department of Medicinal Chemistry,Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China; Tianjin Key Laboratory of Occupational and Environmental Hazard Biomarkers, Tianjin 300162,China zhang78127@tom.com;llzhx@yahoo.cn
  • About author:Xiaojing Qin☆, M.D.,Department of Pathology,Affiliated Hospital of Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162,China

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Abstract:

The alcohol and n-butanol extract of Potentilla anserine L. significantly protects myocardium from acute ischemic injury. However, its effects on rat hippocampal neurons and the mechanism of protection remain unclear. In this study, primary cultured hippocampal neurons from neonatal rats were incubated in 95% N2 and 5% CO2 for 4 hours. Results indicated that hypoxic injury decreased the viability of neurons, increased the expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein. Pretreatment with 0.25, 0.062 5,0.015 6 mg/mL n-butanol extract of Potentilla anserine L. led to a significant increase in cell viability.Expression levels of caspase-9 and caspase-3 mRNA, as well as cytochrome c, Caspase-9, and Caspase-3 protein, were attenuated. The neuroprotective effect of n-butanol extract of Potentilla anserine L. was equivalent to tanshinone IIA. Our data suggest that the n-butanol extract of Potentilla anserine L. could protect primary hippocampal neurons from hypoxic injury by deactivating mitochondrial cell death.