Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (9): 652-658.

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Schisandrin B protects PC12 cells by decreasing the expression of amyloid precursor protein and vacuolar protein sorting 35

Mingmin Yan1, Shanping Mao1, Huimin Dong1, Baohui Liu2, Qian Zhang1, Gaofeng Pan1, Zhiping Fu1   

  1. 1 Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China
    2 Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China
  • Received:2011-09-05 Revised:2012-01-03 Online:2012-03-25 Published:2012-03-25
  • Contact: Shanping Mao, Chief physi-cian, Associate professor, Master’s supervisor, De-partment of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China maospzy@163.com
  • About author:Mingmin Yan★, Master, Department of Neurology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China

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Abstract:

PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer’s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.

Key words: Schisandrin B, PC12 cells, amyloid β-protein 25-35, amyloid precursor protein, vacuolar protein sorting 35, neural protection