Neural Regeneration Research ›› 2014, Vol. 9 ›› Issue (20): 1824-1829.doi: 10.4103/1673-5374.143430

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Three-dimensional conformal intensity-modulated radiation therapy of left femur foci does not damage the sciatic nerve

Wanlong Xu 1, Xibin Zhao 1, Qing Wang 1, Jungang Sun 1, Jiangbo Xu 1, Wenzheng Zhou 1, Hao Wang 1, Shigui Yan 2, Hong Yuan 1   

  1. 1 People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Uygur Autonomous Region, China
    2 The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China
  • Received:2014-07-08 Online:2014-10-25 Published:2014-10-25
  • Contact: Hong Yuan, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830011, Xinjiang Uygur Autonomous Region, China, Doctoryuanhong@sina.com. Shigui Yan, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China, zrjwsj@zju.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81360276; a grant from the Science and Technology Project of Xinjiang Uygur Autonomous Region in China, No. 2013911129; a grant from the Youth Science and Technology Innovation Talents Project of Xinjiang Uygur Autonomous Region-Young Doctor Talents Training Project, No. 2013731013; the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2012211B34; the China Postdoctoral Science Foundation, No. 2014M552566XB.

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Abstract:

During radiotherapy to kill femoral hydatid tapeworms, the sciatic nerve surrounding the focus can be easily damaged by the treatment. Thus, it is very important to evaluate the effects of radiotherapy on the surrounding nervous tissue. In the present study, we used three-dimensional, conformal, intensity-modulated radiation therapy to treat bilateral femoral hydatid disease in Meriones meridiani. The focus of the hydatid disease on the left femur was subjected to radiotherapy (40 Gy) for 14 days, and the right femur received sham irradiation. Hematoxylin-eosin staining, electron microscopy, and terminal deoxynucleotidyl transferase-dUTP nick end labeling assays on the left femurs showed that the left sciatic nerve cell structure was normal, with no obvious apoptosis after radiation. Trypan blue staining demonstrated that the overall protoscolex structure in bone parasitized with Echinococcus granulosus disappeared in the left femur of the animals after treatment. The mortality of the protoscolex was higher in the left side than in the right side. The succinate dehydrogenase activity in the protoscolex in bone parasitized with Echinococcus granulosus was lower in the left femur than in the right femur. These results suggest that three-dimensional conformal intensity-modulated radiation therapy achieves good therapeutic effects on the secondary bone in hydatid disease in Meriones meridiani without damaging the morphology or function of the sciatic nerve.

Key words: nerve regeneration, three-dimensional conformal intensity-modulated radiation therapy, hydatid disease, sciatic nerve, neurons, radiation damage, succinate dehydrogenase, NSFC grants, neural regeneration