Neural Regeneration Research ›› 2017, Vol. 12 ›› Issue (6): 969-976.doi: 10.4103/1673-5374.208592

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Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomotor functions after spinal cord contusion

Zhan-qiong Zhong1, 2, Yang Xiang1, Xi Hu1, You-cui Wang3, Xi Zeng1, Xiao-meng Wang1, Qing-jie Xia3, Ting-hua Wang3, Xiao Zhang1   

  1. 1 Experiment Technology Center of Preclinical Medicine of Chengdu Medical College, Chengdu, Sichuan Province, China; 2 School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China; 3 Institute of Neurological Diseases, Center for Translational Neuroscience, Western China Hospital, Sichuan University, Chengdu, Sichuan Province, China
  • Received:2017-05-05 Online:2017-06-15 Published:2017-06-15
  • Contact: Xiao Zhang, Ph.D., zhangxiao1985007@126.com.
  • Supported by:

    This work was supported by the National Undergraduate Innovation Training Project of China, No. 201313705005.

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Abstract:

Synaptosomal-associated protein 25 kDa (SNAP-25) is localized on the synapse and participates in exocytosis and neurotransmitter release. Decreased expression of SNAP-25 is associated with Alzheimer’s disease and attention deficit/hyperactivity disorder. However, the expression of SNAP-25 in spinal cord contusion injury is still unclear. We hypothesized that SNAP-25 is associated with sensory and locomotor functions after spinal cord injury. We established rat models of spinal cord contusion injury to detect gene changes with a gene array. A decreased level of SNAP-25 was detected by quantitative real time-polymerase chain reaction and western blot assay at 1, 3, 7, 14 and 28 days post injury. SNAP-25 was localized in the cytoplasm of neurons of the anterior and posterior horns, which are involved in locomotor and sensory functions. Our data suggest that reduced levels of SNAP-25 are associated with sensory and locomotor functions in rats with spinal cord contusion injury.

Key words: nerve regeneration, synaptosomal-associated protein 25 kDa, sensory function, locomotor function, spinal cord injury, gene array, neurons, neural regeneration