Abstract: Glaucoma is a leading cause of irreversible blindness, expected to affect 140 million people worldwide by the year 2040. The pathophysiology of glaucoma involves retinal ganglion cell loss, optic nerve atrophy and cupping of the optic disk, culminating in visual field loss (Boia et al., 2020). Glaucoma is a multifactorial disease in which increased intraocular pressure is the main risk factor. Therefore, the majority of experimental models are based on ocular hypertension that mimics in some extend the features of clinical glaucoma (Aires et al., 2017). Elevated hydrostatic pressure is also suitable to disentangle the cellular mechanisms involved in disease progression, using cultured cells or tissues (Aires et al., 2017).