Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (8): 1265-1270.doi: 0.4103/1673-5374.162759

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Bone marrow mesenchymal stem cells transplantation promotes the release of endogenous erythropoietin after ischemic stroke

Wen Lv1, Wen-yu Li1,, Xiao-yan Xu1, Hong Jiang1, Oh Yong Bang2   

  1. 1 Department of Neurology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
    2 Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea
  • Received:2015-07-27 Online:2015-08-24 Published:2015-08-24
  • Contact: Wen-yu Li, M.D., Ph.D., lee_wenyu@zju.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81371258; a grant from the TCM General Research Project of Zhejiang Province of China, No. 2015ZA061; a grant from the Education of Zhejiang Province of China, Y201431639.
     

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Abstract:

This study investigated whether bone marrow mesenchymal stem cell (BMSC) transplantation protected ischemic cerebral injury by stimulating endogenous erythropoietin. The model of ischemic stroke was established in rats through transient middle cerebral artery occlusion. Twenty-four hours later, 1 × 106 human BMSCs (hBMSCs) were injected into the tail vein. Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats. Simultaneously, 3 μg/d soluble erythropoietin receptor (sEPOR) was injected into the lateral ventricle, and on the next 13 consecutive days. sEPOR blocked the release of endogenous erythropoietin. The neurogenesis in the subventricular zone was less in the hBMSCs + sEPOR group than in the hBMSCs + heat-denatured sEPOR group. The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group. The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group. These findings confirm that BMSCs contribute to neurogenesis and improve neurological function by promoting the release of endogenous erythropoietin following ischemic stroke.

 

Key words: nerve regeneration, stem cells, erythropoietin, ischemic stroke, erythropoietin receptor, cell proliferation, cytokine, BrdU, functional recovery, NSFC grant, neural regeneration