Loading...

Table of Content

    24 August 2015, Volume 10 Issue 8 Previous Issue    Next Issue
    For Selected: Toggle Thumbnails
    Curcumin and Apigenin – novel and promising therapeutics against chronic neuroinflammation in Alzheimer’s disease
    Madhuri Venigalla, Erika Gyengesi, Gerald Münch
    2015, 10 (8):  1181-1185.  doi: 10.4103/1673-5374.162686
    Abstract ( 419 )   PDF (201KB) ( 618 )   Save

    Alzheimer’s disease is a progressive neurodegenerative disorder, characterized by deposition of amyloid beta, neurofibrillary tangles, astrogliosis and microgliosis, leading to neuronal dysfunction and loss in the brain. Current treatments for Alzheimer’s disease primarily focus on enhancement of cholinergic transmission. However, these treatments are only symptomatic, and no disease-modifying drug is available for Alzheimer’s disease patients. This review will provide an overview of the proven antioxidant, anti-inflammatory, anti-amyloidogenic, neuroprotective, and cognition-enhancing effects of curcumin and apigenin and discuss the potential of these compounds for Alzheimer’s disease prevention and treatment. We suggest that these compounds might delay the onset of Alzheimer’s disease or slow down its progression, and they should enter clinical trials as soon as possible.

    Related Articles | Metrics
    Neuroimmunomodulatory effects of transcranial laser therapy combined with intravenous tPA administration for acute cerebral ischemic injury
    Philip V. Peplow
    2015, 10 (8):  1186-1190.  doi: 10.4103/1673-5374.162687
    Abstract ( 280 )   PDF (183KB) ( 510 )   Save

    At present, the only FDA approved treatment for ischemic strokes is intravenous administration of tissue plasminogen activator within 4.5 hours of stroke onset. Owing to this brief window only a small percentage of patients receive tissue plasminogen activator. Transcranial laser therapy has been shown to be effective in animal models of acute ischemic stroke, resulting in significant improvement in neurological score and function. NEST-1 and NEST-2 clinical trials in human patients have demonstrated the safety and positive trends in efficacy of transcranial laser therapy for the treatment of ischemic stroke when initiated close to the time of stroke onset. Combining intravenous tissue plasminogen activator treatment with transcranial laser therapy may provide better functional outcomes. Statins given within 4 weeks of stroke onset improve stroke outcomes at 90 days compared to patients not given statins, and giving statins following transcranial laser therapy may provide an effective treatment for patients not able to be given tissue plasminogen activator due to time constraints.

    Related Articles | Metrics
    Nanotechnology and bio-functionalisation for peripheral nerve regeneration
    Tina Sedaghati, Alexander M. Seifalian
    2015, 10 (8):  1191-1194.  doi: 10.4103/1673-5374.162678
    Abstract ( 161 )   PDF (563KB) ( 616 )   Save

    There is a high clinical demand for new smart biomaterials, which stimulate neuronal cell proliferation, migration and increase cell-material interaction to facilitate nerve regeneration across these critical-sized defects. This article briefly reviews several up-to-date published studies using Arginine-Glycine-Aspartic acid peptide sequence, nanocomposite based on polyhedral oligomeric silsesquioxane nanoparticle and nanofibrous scaffolds as promising strategies to enhance peripheral nerve regeneration by influencing cellular behaviour such as attachment, spreading and proliferation. The aim is to establish the potent manipulations, which are simple and easy to employ in the clinical conditions for nerve regeneration and repair.

    Related Articles | Metrics
    A case to be made: theoretical and empirical arguments for the need to consider fatigue in post-stroke motor rehabilitation
    Annette Sterr, Leonardo Furlan
    2015, 10 (8):  1195-1197.  doi: 10.4103/1673-5374.162689
    Abstract ( 336 )   PDF (282KB) ( 595 )   Save

    A case to be made: theoretical and empirical arguments for the need to consider fatigue in post-stroke motor rehabilitation
    Rehabilitation after stroke is essential and represents an important part of the global stroke challenge. Upper limb hemiparesis after stroke is a major problem for both patients and clinicians. Evidence-based practice suggests that constraint-induced movement therapy (CI therapy) is a very effective training-based treatment for upper limb hemiparesis. However, the signature CI therapy protocol requires relatively good levels of residual motor capacity, and is hence not easily applicable to the full range of stroke survivors. Prof. Annette Sterr (University of Surrey, UK) considered that for the past few years, this has prompted investigators to deliver modified versions of the original CI therapy protocol (modified CI therapy), in an attempt to increase the number of stroke survivors that could benefit from this intervention.

    Related Articles | Metrics
    Distribution pattern of axonal cytoskeleton proteins in the human optic nerve head
    Min Hye Kang, Dao-Yi Yu
    2015, 10 (8):  1198-1200.  doi: 10.4103/1673-5374.162691
    Abstract ( 215 )   Save

    Distribution pattern of axonal cytoskeleton proteins in the human optic nerve head拒Glaucoma is one of the leading causes of blindness in developed world. It is progressive optic neuropathy where structural loss of retinal ganglion cell (RGC) axons corresponds with functional visual field defect. Glaucoma is distinguished from other optic neuropathies by its selective loss of RGC axons. Superior and inferior peripheral nerve sectors are found to be most vulnerable to pressure induced injury whereas inner temporal sector is most resilient. Pathogenesis behind the preferential axonal damage pattern is still poorly understood. Prof. Dao-Yi Yu (The University of Western Australia) showed that as RGC axons traverse a long distance through a number of different mechanical and extracellular environments along their projected path, it is expected that the cytoskeleton will play a critical role in maintaining axonal integrity. The distribution of cytoskeleton protein in the optic nerve head may therefore provide valuable information regarding the energetics and vulnerability of axonal injury in each compartment. Axonal cytoskeleton proteins are inherently linked to RGC health and disease. Intermediate filaments, microtubules, actin filament and microtubule associated proteins (MAPs) constitute axonal cytoskeletons. Cytoskeleton protein behaviour is shaped by physiological variables such as absolute tissue pressure and pressure gradients. Additionally, structural determinants of regional cytoskeleton protein concentration include myelin proteins and the pattern of mitochondrial distribution. The variation in mitochondrial organelle, myelin protein content and neural tissue pressure along the length of the human RGC axon could potentially influence the regional concentration of cytoskeleton protein subunits. This may help explain why the optic nerve head is injured in an asymmetrical fashion in many diseases including glaucoma.

    Related Articles | Metrics
    The neuroprotective effects of human growth hormone as a potential treatment for amyotrophic lateral sclerosis
    Jin-Young Chung, Jun-Sang Sunwoo, Min-Wook Kim, Manho Kim
    2015, 10 (8):  1201-1203.  doi: 10.4103/1673-5374.162690
    Abstract ( 254 )   PDF (185KB) ( 743 )   Save

    The Neuroprotective Effects of Growth Hormone Growth hormone (GH) is a single-chain polypeptide with 191 amino acids that mainly controls various physiologic processes such as growth and metabolism. GH binds to the membrane receptors of target cells such as adipocyte and chondrocyte, and directly stimulates proliferation. In addition, GH can act indirectly through the synthesis of insulin-like growth factor 1 (IGF-1) in the liver and target tissue. IGF-1 actually exerts growth promoting effects on a wide range of tissue, and leads to the bone and muscle growth. Regarding to the control of metabolism, GH promotes protein anabolism, mobilization of stored triglyceride, hepatic glucose production, and insulin resistance. This perspective wrote by Prof. Manho Kim's team (Seoul National University Hospital) describes the signal transduction pathway, physiological functions in the brain, and the neuroprotective effects of GH with focus on its therapeutic potential in amyotrophic lateral sclerosis (ALS).

    Related Articles | Metrics
    The vascular stem cell niche: roadmap for transplanted neural progenitor cells during environmental enrichment?
    Ben Waldau
    2015, 10 (8):  1204-1205.  doi: 10.4103/1673-5374.162692
    Abstract ( 228 )   PDF (547KB) ( 568 )   Save

    The vascular stem cell niche: roadmap for transplanted neural progenitor cells during environmental enrichment?
    One of the challenges of cell transplantation into the brain is poor graft survival. Graft survival may be affected by an immunological response of the host towards transplanted cells, shear injury to cells during transplantation or an unsuitable micro-environment for the transplanted cell type. Prof. Ben Waldau (UC Davis Medical Center, USA) proposed that neural progenitor cells have an affinity to laminin, which is commonly used to maintain neural progenitorsas monolayer cultures in vitro. The affinity of neural progenitor cells to laminin is based on thehigh expression ofα6β1 integrin, which is required for binding of neural progenitors to endothelial cells. They observed that transplanted neural progenitor cells are closely associated with the vascular stem cell niche and responsive to environmental enrichment. Therefore, survival of neural progenitor cell grafts in humans may be enhanced by physical, occupational or speech therapy to increase regional brain perfusion and help promote survival of transplanted cells in desired target areas. Further research is needed to study whether the association of transplanted dentate progenitor cells with the vascular stem cell niche and the responsiveness to environmental enrichment persists over time.

    Related Articles | Metrics
    Localized regulation of the axon shaft during the emergence of collateral branches
    Gianluca Gallo
    2015, 10 (8):  1206-1208.  doi: 10.4103/1673-5374.162694
    Abstract ( 257 )   PDF (838KB) ( 807 )   Save

    The ability of the axon to form de novo collateral branches along its length is fundamental to the establishment of complex patterns of connectivity during development and is also a major response of many axonal populations following injury. The emergence of branches is under both positive and negative control by extracellular signals. How the site of branch formation is determined is a fundamental question regarding the formation of branches. In theory, the whole axon shaft has the potential to give rise to a branch, but yet branches form only at specific sites. This feature of the formation of branches is emphasized by studies in which nerve growth factor (NGF), covalently attached to 10 micron beads, was applied locally along embryonic sensory axons. Although contact of the beads with the axon shaft was able to drive the formation of a branch at the sites of contact, even after 3 hours of continuous contact only 46% of sites gave rise to a branch. Similarly, when NGF is bath applied to cultured sensory neurons, and thus the whole surface of the axon is exposed to NGF, axons usually only generate a maximum of 4-6 branches along the distal 100 microns of the axon, a response that is maximal by 30 min of treatment. Recent work, from our laboratory and others, has begun to shed light on why axons form branches at specific sites.

    Related Articles | Metrics
    Ubiquitin homeostasis: from neural stem cell differentiation to neuronal development
    Han-Wook Ryu, Kwon-Yul Ryu
    2015, 10 (8):  1209-1210.  doi: 10.4103/1673-5374.162693
    Abstract ( 369 )   PDF (309KB) ( 527 )   Save

    Ubiquitin homeostasis: From neural stem cell differentiation to neuronal development Ubiquitin (Ub) is a well-known eukaryotic protein that exerts diverse signaling functions inside the cells, including targeting its substrates for proteasomal degradation. Based on Prof. Kwon-Yul Ryu et al.' (University of Seoul, Republic of Korea) recent reports, they speculate that measuring cellular levels of free Ub will be essential for the optimal differentiation of neural stem cells into neurons and for neuronal development. Ub may be one of the key proteins in determining the fate of neural stem cells. Thus, Ub homeostasis should be considered to maximize the efficacy of neural stem cell transplantation. Therefore, their research represents a milestone in the development of a novel and universal therapeutic strategy to overcome various neurodegenerative diseases.

    Related Articles | Metrics
    The neuroprotective potential of endoplasmic reticulum chaperones
    Todd McLaughlin, Sarah X. Zhang
    2015, 10 (8):  1211-1213.  doi: 10.4103/1673-5374.162696
    Abstract ( 178 )   PDF (484KB) ( 739 )   Save

     The molecular chaperones and the manipulation of the UPR pathways have exciting therapeutic potential as neuroprotectants at the cellular level for a variety of neurodegenerative diseases. Additionally, the importance of endoplasmic reticulum chaperones in regulating the production and homeostasis of neurotrophic factors through autocrine, paracrine, and endocrine signaling is worth pursuing. Furthermore, the implications of ER chaperone manipulation in the development and regeneration of neurons in the retina as well as other neural systems is potentially a very promising area for future study. All in all, in-depth understanding of the functions, in particular the neuroprotective potential, of ER chaperones in healthy and stressed neural tissues may provide an intriguing approach in managing multiple disease types to preserve long-term functionality.

    Related Articles | Metrics
    Fractalkine: multiple strategies to counteract glutamate receptors activation leading to neuroprotection
    Clotilde Lauro
    2015, 10 (8):  1214-1215.  doi: 10.4103/1673-5374.162697
    Abstract ( 257 )   PDF (143KB) ( 524 )   Save

    Glutamate (Glu) is the main excitatory amino acid in the brain and plays a pivotal role in many neurophysiological functions. Nevertheless, an excess and prolonged exposure to Glu determines the overactivation of glutamate receptors (GluRs) with consequent impairment of cellular calcium (Ca2+) homeostasis, leading to the dysregulation of intracellular pathways and resulting in neuronal dysfunction and death, a process called excitotoxicity. In the last two decades, excitotox mechanisms have been proposed to explain the neuronal cell death characteristic of neurodegenerative diseases such as Huntington’s, Alzheimer’s and Parkinson’s disease, including increase of intracellular Ca2+, accumulation of oxidizing free radicals, impairment of mitochondrial function and activation of apoptotic and autophagic programs. Since excitotoxicity is implicated in a variety of neuropathological conditions it represents a common pathogenic pathway for neurodegenerative diseases with distinct genetic etiologies and it appears to be important in determining the extent of tissue damage.
    Dr. Clotilde Lauro (Sapienza University of Rome, Italy) showed that in the attempt to counteract excitotoxic insult, damaged neurons respond by releasing soluble factors that might be sensed by surrounding cells to induce a wide range of cellular responses leading to neuroprotection and tissue damage repair. Among these factors there is fractalkine (CX3CL1), a chemokine constitutively expressed on neuronal membrane that is upregulated, cleaved and released upon excitotoxic insult.

    Related Articles | Metrics
    A useful electroencephalography (EEG) marker of brain plasticity: delta waves
    Giovanni Assenza, Vincenzo Di Lazzaro
    2015, 10 (8):  1216-1217.  doi: 10.4103/1673-5374.162698
    Abstract ( 351 )   PDF (293KB) ( 758 )   Save

    EEG is a reliable technique for exploring functional activity of the brain and is sensitive to changes in neural plasticity processes related to LTP-like phenomena and clinical recovery after brain lesions. Its use in this field of neuroscience should be encouraged, in particular in searching traces of plasticity processes to provide a biological marker to lead improvements of brain functioning.

    Related Articles | Metrics
    Combining motor learning and brain stimulation to enhance post-stroke neurorehabilitation
    Yves Vandermeeren, Stéphanie Lefebvre
    2015, 10 (8):  1218-1220.  doi: 10.4103/1673-5374.158483
    Abstract ( 185 )   PDF (285KB) ( 455 )   Save

    Combining motor learning and brain stimulation to enhance post-stroke neurorehabilitation Worldwide, stroke is a leading cause of life-long disability resulting in dramatic restrictions in patient’s independence and in a growing economic burden for the community. The majority of stroke survivors suffers from chronic sequels among which hemiparesis is one of the most debilitating. Despite quick progresses over the last 20 years, the impact of neurorehabilitation on post-stroke recovery remains unsatisfactory. Developing new ways to enhance neurorehabilitation could thus benefit to millions of patients. Prof. Yves Vandermeeren (Université catholique de Louvain, Belgium) demonstrated that a better insight into the physiology of the normal motor system and the mechanisms driving post-stroke recovery and neural plasticity should permit to develop a new science of neurorehabilitation.

    Related Articles | Metrics
    The neuroprotective effects of (S)-3,5-dihydroxyphenylglycine preconditioning in middle cerebral artery occluded rats: a perspective as a contrivance for stroke
    Nik Nasihah Nik Ramli, Rosfaiizah Siran
    2015, 10 (8):  1221-1222.  doi: 10.4103/1673-5374.162748
    Abstract ( 193 )   PDF (209KB) ( 618 )   Save
    Due to the nature of neuron which is irreversible to damage, ischemic preconditioning is unlikely to give any benefit to ischemic stroke patients. However, preconditioning is an appealing avenue for surgical procedures which predisposed the patients with higher risk of ischemic brain injury such as endarterectomy and cerebral aneurysm surgery. We demonstrated that preconditioning with lower doses of (S)-3,5-DHPG showed to be protective against subsequent ischemic insult in the acute ischemic stroke rats. Therefore, understanding of mechanisms underlying the preconditioning effect of (S)-3,5-DHPG is a critical point of salvation in this area.
     
    Related Articles | Metrics
    Nogo-A and its functions beyond axonal inhibition: the controversial role of Nogo-A in Parkinson’s disease
    Stefanie Seiler, Hans R. Widmer
    2015, 10 (8):  1223-1224.  doi: 10.4103/1673-5374.162749
    Abstract ( 456 )   PDF (612KB) ( 589 )   Save

    The role and involvement of Nogo-A in Parkinson’s disease (PD) is just beginning to evolve. Even though none of the Nogo-A signaling genes has been associated with PD, the results from animal studies showing that DAergic neurons co-expressing Nogo-A survive better in the later course of PD as well as more pronounced DAergic cell death in PD Nogo-A knock-out animals hint strongly to a neuroprotective role of Nogo-A in DAergic neurons. Moreover, Nogo-A knock-out mice have a better motor coordination as well as an enhanced locomotor reaction to systemic amphetamine injections compared to wild type mice. The function of Nogo-A in DAergic neurons, however, needs to be further elucidates especially with focus on its signaling. After all, other studies showed that LINGO-1 is increased in animal models of PD and hampered the survival of DAergic neurons. Moreover, antagonization of NgR1 or LINGO-1 led to significant increase in survival of DAgeric neurons, showing that the NgR1 complex has a negative regulatory role on the survival of DAergic neurons. Considering these findings, the potential neuroprotective role of Nogo-A in PD is probably not due to the Nogo-66 domain that signals through the NgR1 complex. Yet, if and how Nogo-A protects DAergic neurons from toxic insults and if there are other myelin associated proteins that act through the NgR1complex as negative regulator of DAergic neuron survival needs to be further investigated.

    Related Articles | Metrics
    A new mechanism for protection of dopaminergic neurons mediated by astrocytes
    Juan Segura-Aguilar
    2015, 10 (8):  1225-1227.  doi: 10.4103/1673-5374.162750
    Abstract ( 194 )   PDF (236KB) ( 532 )   Save

    A new mechanism for dopaminergic neurons protection mediated by astrocytes For a long time, the question about the mechanism involved in the degenerative process of the nigrostriatal system in Parkinson’s disease (PD), resulting in the loss of dopaminergic neurons containing neuromelanin, has remained open. The discovery of genes associated with familial forms of PD, such as α-synuclein (SNCA), parkin, DJ-1, PINK-1, LRRK-2, ATP13A2, PINK-1 and others resulted in important input into the basic research in this field with the aim of understanding the role of these proteins in sporadic PD. In the scientific community, there is a general agreement that the loss of dopaminergic neurons containing neuromelanin in the nigrostriatal system involves mitochondria dysfunction, protein degradation dysfunction, and SNCA aggregation in neurotoxic oligomers, oxidative stress, neuroinflammation and endoplasmic reticulum stress.
    Prof. Juan Segura-Aguilar (University of Chile, Chile) proposed that the neurotoxin involved in the degenerative process of the dopaminergic nigrostriatal system must be of endogenous origin, since the progression of the neurodegenerative process in PD is very slow and takes years for the development of motor symptoms, when up to 60–70% of dopaminergic neurons containing neuromelanin are lost. The slow progression of the degenerative process in PD contrasts with the extremely rapid degeneration observed in humans who are injected with drugs containing 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), who after only 3 days develop severe motor symptoms. The possibility that o-quinones derived from dopamine oxidation are responsible for the loss of dopaminergic neurons containing neuromelanin in PD is supported by the fact that these neurons require dopamine oxidation to o-quinones to generate neuromelanin. Interestingly, o-quinones generated during dopamine oxidation have been reported to induce mitochondria dysfunction, SNCA aggregation in neurotoxic oligomers, protein degradation dysfunction of both proteasomal and lysosomal systems and oxidative stress.

    Related Articles | Metrics
    Identifying specific RGC types may shed light on their idiosyncratic responses to neuroprotection
    Manuel Vidal-Sanz, Francisco M. Nadal-Nicolás, Francisco J. Valiente-Soriano, Marta Agudo-Barriuso, Maria P. Villegas-Pérez
    2015, 10 (8):  1228-1230.  doi: 10.4103/1673-5374.162751
    Abstract ( 228 )   PDF (522KB) ( 576 )   Save

    Identifying specific RGC types may shed light on their idiosyncratic responses to neuroprotection Retinal ganglion cells (RGCs) are located in the innermost layer of the retina and are the only output retinal neurons, conveying light information to the main retinorecipient target regions of the brain responsible for the image and non-image forming visual functions. There are well over twenty RGC types, each with its own dendritic morphological and physiological characteristics, target territories and visual functions. The study of the responses of RGCs to various injury-induced or inherited retinal degenerations requires the identification of these neurons. Up to know RGCs have been identified mainly with retrograde tracers applied to their principal retinorecipient target nuclei in the brain, the superior colliculi (SCi), or to their main axonal output, the optic nerve (ON). These methods, although most efficient to label the great majority of RGCs do not allow distinction between different RGC types. Retrogradely transported tracers applied to the ON result in massive retrograde labelling of RGCs, while tracer application to the SCi labels most RGCs projecting to these nuclei as well as to neighbouring areas. Indeed, injection into the optic tract or tracer application over the SCi may result in spurious labelling of neurons in the brain located in different neighbour areas. Prof. Manuel Vidal-Sanz (Universidad de Murcia, Spain) considered that new discoveries of suitable molecular markers to identify other types of RGCs would allow deciphering and dissecting out the responses of RGCs and thus further advance our knowledge of the molecular mechanisms in each of these types that render them susceptible and/or capable of survival, amenable to rescuing intervention or axonal regeneration. Indeed, new discoveries or characterization of molecular markers for specific types of RGCs may allow further characterization of the responses of different types of RGCs to axonal injury, such as neuronal survival, axonal regeneration, synapse formation and re-establishment of function.

    Related Articles | Metrics
    A new tool for monitoring brain function: eye tracking goes beyond assessing attention to measuring central nervous system physiology
    Uzma Samadani
    2015, 10 (8):  1231-1233.  doi: 10.4103/1673-5374.162752
    Abstract ( 172 )   PDF (431KB) ( 557 )   Save

    A new tool for monitoring brain function: eye tracking goes beyond assessing attention to measuring central nervous system physiology
    Concussion and other forms of brain injury, elevated intracranial pressure, dementia and myriadother function-impairing neurologic conditions are not always detectable with conventional means. The lack of accurate diagnostics, biomarkers, and outcome measures has a devastating impact. Individual patients may suffer in obscurity, self-medicate into an addictive spiral, have impaired professional activity, and develop failed interpersonal relationships. Without accurate diagnostics it is impossible to know the incidence of the problem or assess its societal impact. The lack of appropriate classification schemes and objective outcome measures for patients entering clinical trials for concussion and other forms of traumatic brain injury (TBI) contributes to the failure of such trials for therapeutics and prophylactics at great expense to the research and development community and those it hopes to serve, including athletes, students and hapless victims of trauma. Recently Dr. Uzma Samadani’s group published two manuscripts describing a novel algorithm for eye tracking that will be useful for concussion, other forms of TBI and other neuropathologies. Eye tracking assesses brain function rather than appearance or electrical activity, and thus represents a relatively newer modality for assessment of central nervous system integrity. The difference between the two papers published by their group and nearly all prior eye tracking publications, is that these new papers utilize non-spatially calibrated eye tracking. Rather than assess what someone chooses to look at, the tracking measures how well the eyes are capable of moving.

    Related Articles | Metrics
    Central plasticity resulting from chronic low back pain in degenerative disorders of the spine.
    Michael Luchtmann, Raimund Firsching
    2015, 10 (8):  1234-1236.  doi: 10.4103/1673-5374.162754
    Abstract ( 190 )   PDF (382KB) ( 560 )   Save

    Central plasticity resulting from chroniclow back pain in degenerative disorders of the spine Previous findings have shown that intervertebral disc herniation can occur in people who are asymptomatic. However, much effort has been expended to identify prognostic variables based on the classification of the magnetic resonance imaging (MRI) of the spine. Unfortunately, no factor has yet been revealed that reliably distinguishes between patients who should be treated conservatively and those who would instead benefit from surgery. In fact, only a weak correlation has been observed between the size of the prolapsed disc and the presence of clinical symptoms. Since it is the brain that ultimately interprets pain, the neuroscientific community has in recent years increased its focus on studying pain-induced cerebral alterations. And indeed, the adult human brain has an astonishing capacity for the morphological alterations that follow the learning and adaptation processes necessary to a changed environment. Dr. Michael Luchtmann (Otto-von-Guericke-University Magdeburg, Germany) showed that until recently, chronic pain was thought to be associated with abnormal nociceptive function but an unchanged brain structure. Now, however, a large body of new evidence supports the idea that chronic pain not only signals an altered functional state but is also a consequence of central plasticity.

    Related Articles | Metrics
    Aminoacyl tRNA synthetases and their relationships with peripheral nerve degeneration and regeneration
    Junyang Jung
    2015, 10 (8):  1237-1238.  doi: 10.4103/1673-5374.162753
    Abstract ( 230 )   PDF (290KB) ( 516 )   Save
    Following damage resulting from mechanical injury, viral infection, or autoimmunity, peripheral nerves degenerate and a variety of complications, including sensory loss, muscular paralysis, skin thinning, and a loss of tendon reflexes, can manifest. If these complications persist, they can cause a number of debilitating personal and/or social problems. For example, Guillain-Barre syndrome is induced by the degradation of myelin sheaths, and if the symptoms are not controlled the disease can be life-threatening due to the demyelination of respiratory muscle nerves. To date, effective treatment strategies that can inhibit the demyelination of peripheral nerves in the early stages of disease have yet to be developed. Thus, as with severe cases of Guillain-Barre syndrome, devices that alleviate the symptoms of respiratory muscular paralysis, including breathing machines, must be utilized until a cure can be developed.
     
    Related Articles | Metrics
    Neuroprotective effects of atorvastatin against cerebral ischemia/reperfusion injury through the inhibition of endoplasmic reticulum stress
    Jian-wen Yang, Zhi-ping Hu
    2015, 10 (8):  1239-1244.  doi: 10.4103/1673-5374.162755
    Abstract ( 184 )   PDF (1124KB) ( 572 )   Save

    Cerebral ischemia triggers secondary ischemia/reperfusion injury and endoplasmic reticulum stress initiates cell apoptosis. However, the regulatory mechanism of the signaling pathway remains unclear. We hypothesize that the regulatory mechanisms are mediated by the protein kinase-like endoplasmic reticulum kinase/eukaryotic initiation factor 2α in the endoplasmic reticulum stress signaling pathway. To verify this hypothesis, we occluded the middle cerebral
    artery in rats to establish focal cerebral ischemia/reperfusion model. Results showed that the expression levels of protein kinase-like endoplasmic reticulum kinase and caspase-3, as well as the phosphorylation of eukaryotic initiation factor 2α, were increased after ischemia/reperfusion. Administration of atorvastatin decreased the expression of protein kinase-like endoplasmic reticulum kinase, caspase-3 and phosphorylated eukaryotic initiation factor 2α, reduced the infarct volume and improved ultrastructure in the rat brain. After salubrinal, the specific inhibitor of phosphorylated eukaryotic initiation factor 2α was given into the rats intragastrically, the expression levels of caspase-3 and phosphorylated eukaryotic initiation factor 2α in the were decreased, a reduction of the infarct volume and less ultrastructural damage were observed than the untreated, ischemic brain. However, salubrinal had no impact on the expression of protein kinase-like endoplasmic reticulum kinase. Experimental findings indicate that atorvastatin inhibits endoplasmic reticulum stress and exerts neuroprotective effects. The underlying mechanisms of attenuating ischemia/reperfusion injury are associated with the protein kinase-like endoplasmic reticulum kinase/eukaryotic initiation factor 2α/caspase-3 pathway.

    Related Articles | Metrics
    Exercise preconditioning exhibits neuroprotective effects on hippocampal CA1 neuronal damage after cerebral ischemia
    Nabi Shamsaei, Mehdi Khaksari, Sohaila Erfani, Hamid Rajabi, Nahid Aboutaleb
    2015, 10 (8):  1245-1250.  doi: 10.4103/1673-5374.162756
    Abstract ( 187 )   PDF (1075KB) ( 844 )   Save

    Recent evidence has suggested the neuroprotective effects of physical exercise on cerebral ischemic injury. However, the role of physical exercise in cerebral ischemia-induced hippocampal damage remains controversial. The aim of the present study was to evaluate the effects of pre-ischemia treadmill training on hippocampal CA1 neuronal damage after cerebral ischemia. Male adult rats were randomly divided into control, ischemia and exercise + ischemia groups. In the exercise + ischemia group, rats were subjected to running on a treadmill in a designated time schedule (5 days per week for 4 weeks). Then rats underwent cerebral ischemia induction through occlusion of common carotids followed by reperfusion. At 4 days after cerebral ischemia, rat learning and memory abilities were evaluated using passive avoidance memory test and rat hippocampal neuronal damage was detected using Nissl and TUNEL staining. Pre-ischemic exercise significantly reduced the number of TUNEL-positive cells and necrotic cell death in the hippocampal CA1 region as compared to the ischemia group. Moreover, pre-ischemic exercise significantly prevented ischemia-induced memory dysfunction. Pre-ischemic exercise mighct prevent memory deficits after cerebral ischemia through rescuing hippocampal CA1 neurons from ischemia-induced degeneration.

    Related Articles | Metrics
    Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction
    Ji Yun Ahn, Hyun-Jin Tae, Jeong-Hwi Cho, In Hye Kim, Ji Hyeon Ahn, Joon Ha Park, Dong Won Kim, Jun Hwi Cho
    2015, 10 (8):  1251-1257.  doi: 10.4103/1673-5374.162757
    Abstract ( 178 )   PDF (1913KB) ( 505 )   Save

    c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction. 

    Related Articles | Metrics
    Activation of the Notch signaling pathway promotes neurovascular repair after traumatic brain injury
    Qi-shan Ran, Yun-hu Yu, Xiao-hong Fu, Yuan-chao Wen
    2015, 10 (8):  1258-1264.  doi: 10.4103/1673-5374.162758
    Abstract ( 223 )   PDF (934KB) ( 883 )   Save

    The Notch signaling pathway plays a key role in angiogenesis and endothelial cell formation, but it remains unclear whether it is involved in vascular repair by endothelial progenitor cells after traumatic brain injury. Therefore, in the present study, we controlled the Notch signaling pathway using overexpression and knockdown constructs. Activation of the Notch signaling pathway by Notch1 or Jagged1 overexpression enhanced the migration, invasiveness and angiogenic ability of endothelial progenitor cells. Suppression of the Notch signaling pathway with Notch1 or Jagged1 siRNAs reduced the migratory capacity, invasiveness and angiogenic ability of endothelial progenitor cells. Activation of the Notch signaling pathway in vivo in a rat model of mild traumatic brain injury promoted neurovascular repair. These findings suggest that the activation of the Notch signaling pathway promotes blood vessel formation and tissue repair after brain trauma.

    Related Articles | Metrics
    Bone marrow mesenchymal stem cells transplantation promotes the release of endogenous erythropoietin after ischemic stroke
    Wen Lv, Wen-yu Li, Xiao-yan Xu, Hong Jiang, Oh Yong Bang
    2015, 10 (8):  1265-1270.  doi: 0.4103/1673-5374.162759
    Abstract ( 188 )   PDF (644KB) ( 627 )   Save

    This study investigated whether bone marrow mesenchymal stem cell (BMSC) transplantation protected ischemic cerebral injury by stimulating endogenous erythropoietin. The model of ischemic stroke was established in rats through transient middle cerebral artery occlusion. Twenty-four hours later, 1 × 106 human BMSCs (hBMSCs) were injected into the tail vein. Fourteen days later, we found that hBMSCs promoted the release of endogenous erythropoietin in the ischemic region of rats. Simultaneously, 3 μg/d soluble erythropoietin receptor (sEPOR) was injected into the lateral ventricle, and on the next 13 consecutive days. sEPOR blocked the release of endogenous erythropoietin. The neurogenesis in the subventricular zone was less in the hBMSCs + sEPOR group than in the hBMSCs + heat-denatured sEPOR group. The adhesive-removal test result and the modified Neurological Severity Scores (mNSS) were lower in the hBMSCs + sEPOR group than in the heat-denatured sEPOR group. The adhesive-removal test result and mNSS were similar between the hBMSCs + heat-denatured sEPOR group and the hBMSCs + sEPOR group. These findings confirm that BMSCs contribute to neurogenesis and improve neurological function by promoting the release of endogenous erythropoietin following ischemic stroke.

     
    Related Articles | Metrics
    The key target of neuroprotection after the onset of ischemic stroke: secretory pathway Ca2+-ATPase 1
    Li-hua Li, Xiang-rong Tian, Zhi-ping Hu
    2015, 10 (8):  1271-1278.  doi: 10.4103/1673-5374.162760
    Abstract ( 181 )   PDF (1533KB) ( 894 )   Save

    The regulatory mechanisms of cytoplasmic Ca2+ after myocardial infarction-induced Ca2+ overload involve secretory pathway Ca2+-ATPase 1 and the Golgi apparatus and are well understood. However, the effect of Golgi apparatus on Ca2+ overload after cerebral ischemia and reperfusion remains unclear. Four-vessel occlusion rats were used as animal models of cerebral ischemia. The expression of secretory pathway Ca2+-ATPase 1 in the cortex and hippocampus was detected by immunoblotting, and Ca2+ concentrations in the cytoplasm and Golgi vesicles were determined. Results showed an overload of cytoplasmic Ca2+ during ischemia and reperfusion that reached a peak after reperfusion. Levels of Golgi Ca2+ showed an opposite effect. The expression of Golgi-specific secretory pathway Ca2+-ATPase 1 in the cortex and hippocampus decreased before ischemia and reperfusion, and increased after reperfusion for 6 hours. This variation was similar to the alteration of calcium in separated Golgi vesicles. These results indicate that the Golgi apparatus participates in the formation and alleviation of calcium overload, and that secretory pathway Ca2+-ATPase 1 tightly responds to ischemia and reperfusion in nerve cells. Thus, we concluded that secretory pathway Ca2+-ATPase 1 plays an essential role in cytosolic calcium regulation and its expression can be used as a marker of Golgi stress, responding to cerebral ischemia and reperfusion. The secretory pathway Ca2+-ATPase 1 can be an important neuroprotective target of ischemic stroke.

    Related Articles | Metrics
    Dantrolene enhances the protective effect of hypothermia on cerebral cortex neurons
    Sui-yi Xu, Feng-yun Hu, Li-jie Ren, Lei Chen, Zhu-qing Zhou, Xie-jun Zhang, Wei-ping Li
    2015, 10 (8):  1279-1285.  doi: 10.4103/1673-5374.162761
    Abstract ( 310 )   PDF (1737KB) ( 818 )   Save

    Therapeutic hypothermia is the most promising non-pharmacological neuroprotective strategy against ischemic injury. However, shivering is the most common adverse reaction. Many studies have shown that dantrolene is neuroprotective in in vitro and in vivo ischemic injury models. In addition to its neuroprotective effect, dantrolene neutralizes the adverse reaction of hypothermia. Dantrolene may be an effective adjunctive therapy to enhance the neuroprotection of hypothermia in treating ischemic stroke. Cortical neurons isolated from rat fetuses were exposed to 90 minutes of oxygen-glucose deprivation followed by reoxygenation. Neurons were treated with 40 μM dantrolene, hypothermia (at 33°C), or the combination of both for 12 hours. Results revealed that the combination of dantrolene and hypothermia increased neuronal survival and the mitochondrial membrane potential, and reduced intracellular active oxygen cytoplasmic histone-associated DNA fragmentation, and apoptosis. Furthermore, improvements in cell morphology were observed. The combined treatment enhanced these responses compared with either treatment alone. These findings indicate that dantrolene may be used as an effective adjunctive therapy to enhance the neuroprotective effects of hypothermia in ischemic stroke.

    Related Articles | Metrics
    The E3 ubiquitin ligase seven in absentia homolog 1 may be a potential new therapeutic target for Parkinson’s disease
    Zeng-lin Cai, Jing Xu, Shou-ru Xue, Yuan-yuan Liu, Yong-jin Zhang, Xin-zhi Zhang, Xuan Wang, Fang-ping Wu, Xiao-min Li
    2015, 10 (8):  1286-1291.  doi: 10.4103/1673-5374.162763
    Abstract ( 297 )   PDF (1133KB) ( 803 )   Save

    In this study, we investigated the effect of an antibody against E3 ubiquitin ligase seven in absentia homolog 1 (SIAH-1) in PC12 cells. 1-Methyl-4-phenylpyridinium (MPP+) treatment increased α-synuclein, E1 and SIAH-1 protein levels in PC12 cells, and it reduced cell viability; however, there was no significant change in light chain 3 expression. Treatment with an SIAH-1 antibody decreased mRNA expression levels of α-synuclein, light chain 3 and SIAH-1, but increased E1 mRNA expression. It also increased cell viability. Combined treatment with MPP+ and rapamycin reduced SIAH-1 and α-synuclein levels. Treatment with SIAH-1 antibody alone diminished α-synuclein immunoreactivity in PC12 cells, and reduced the colocalization of α-synuclein and light chain 3. These findings suggest that the SIAH-1 antibody reduces the monoubiquitination and aggregation of α-synuclein, promoting its degradation by the ubiquitin-proteasome pathway. Consequently, SIAH-1 may be a potential new therapeutic target for Parkinson’s disease.

    Related Articles | Metrics
    Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42
    Zi-hao Liang, Xiao-hui Cheng, Zhi-gang Ruan, Han Wang, Shan-shan Li, Jing Liu, Guo-ying Li, Su-min Tian
    2015, 10 (8):  1292-1297.  doi: 10.4103/1673-5374.162762
    Abstract ( 178 )   PDF (1161KB) ( 998 )   Save

    The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10–10 M and 1 × 10–5 M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10–6 M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

    Related Articles | Metrics
    Electroacupuncture pretreatment exhibits anti-depressive effects by regulating hippocampal proteomics in rats with chronic restraint stress
    Zhuo Guo, Ya Tu, Tian-wei Guo1, Yun-chu Wu, Xue-qin Yang, Lan Sun, Xin-jing Yang, Wen-yue Zhang, Yu Wang, Xu-hui Zhang
    2015, 10 (8):  1298-1304.  doi: 10.4103/1673-5374.162764
    Abstract ( 239 )   PDF (423KB) ( 721 )   Save
    The clinical effect of electroacupuncture on depression is widely recognized. However, the signal transduction pathways and target proteins involved remain unclear. In the present study, rat models of chronic restraint stress were used to explore the mechanism by which electroacupuncture alleviates depression. Rats were randomly divided into control, model, and electroacupuncture groups. Chronic restraint stress was induced in the model and electroacupuncture groups by restraining rats for 28 days. In the electroacupuncture group, electroacupuncture pretreatment at Baihui (GV20) and Yintang (GV29) acupoints was performed daily (1 mA, 2 Hz, discontinuous wave, 20 minutes) prior to restraint for 28 days. Open field tests and body weight measurements were carried out to evaluate the depressive symptoms at specific time points. On day 28, the crossing number, rearing number, and body weights of the model group were significantly lower than those in the control group. Behavior test results indicated that rat models of depressive-like symptoms were successfully established by chronic restraint stress combined with solitary raising. On day 28, an isobaric tag for a relative and absolute quantitation-based quantitative proteomic approach was performed to identify differentially expressed proteins in hippocampal samples obtained from the model and electroacupuncture groups. The potential function of these differential proteins was predicted through the use of the Cluster of Orthologous Groups of proteins (COG) database. Twenty-seven differential proteins (uncharacteristic proteins expected) were selected from the model and electroacupuncture groups. In addition to unknown protein functions, COG are mainly concentrated in general prediction function, mechanism of signal transduction, amino acid transport and metabolism groups. This suggests that electroacupuncture improved depressive-like symptoms by regulating differential proteins, and most of these related proteins exist in nerve cells.
     
    Related Articles | Metrics
    Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation
    Ya-jing Zhou, Jian-min Liu, Shu-ming Wei, Yun-hao Zhang, Zhen-hua Qu, Shu-bo Chen
    2015, 10 (8):  1305-1311.  doi: 10.4103/1673-5374.162765
    Abstract ( 201 )   PDF (1965KB) ( 682 )   Save

    Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

    Related Articles | Metrics
    Differentiation of Wharton’s jelly mesenchymal stem cells into neurons in alginate scaffold
    Seyed Mojtaba Hosseini, Attiyeh Vasaghi, Newsha Nakhlparvar, Reza Roshanravan, Tahereh Talaei-khozani, Zahra Razi
    2015, 10 (8):  1312-1316.  doi: 10.4103/1673-5374.162768
    Abstract ( 259 )   PDF (962KB) ( 897 )   Save

    Alginate scaffold has been considered as an appropriate biomaterial for promoting the differentiation of embryonic stem cells toward neuronal cell lineage. We hypothesized that alginate scaffold is suitable for culturing Wharton’s jelly mesenchymal stem cells (WJMSCs) and can promote the differentiation of WJMSCs into neuron-like cells. In this study, we cultured WJMSCs in a three-dimensional scaffold fabricated by 0.25% alginate and 50 mM CaCl2 in the presence of neurogenic medium containing 10 μM retinoic acid and 20 ng/mL basic fibroblast growth factor. These cells were also cultured in conventional two-dimensional culture condition in the presence of neurogenic medium as controls. After 10 days, immunofluorescence staining was performed for detecting β-tubulin (marker for WJMSCs-differentiated neuron) and CD271 (motor neuron marker). β-Tubulin and CD271 expression levels were significantly greater in the WJMSCs cultured in the three-dimensional alginate scaffold than in the conventional two-dimensional culture condition. These findings suggest that three-dimensional alginate scaffold cell culture system can induce neuronal differentiation of WJMSCs effectively.

    Related Articles | Metrics
    Panax notoginseng saponins improve recovery after spinal cord transection by upregulating neurotrophic factors
    Bo Wang, Yu Li, Xuan-peng Li, Yang Li
    2015, 10 (8):  1317-1320.  doi: 10.4103/1673-5374.162766
    Abstract ( 170 )   PDF (203KB) ( 601 )   Save

    Saponins extracted from Panax notoginseng are neuroprotective, but the mechanisms underlying this effect remain unclear. In the present study, we established a rat model of thoracic (T10) spinal cord transection, and injected Panax notoginseng saponins (100 mg/kg) or saline 30 minutes after injury. Locomotor functions were assessed using the Basso, Beattie, and Bresnahan (BBB) scale from 1 to 30 days after injury, and immunohistochemistry was carried out in the ventral horn of the spinal cord at 1 and 7 days to determine expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). Our results show that at 7–30 days post injury, the BBB score was higher in rats treated with Panax notoginseng saponins than in those that received saline. Furthermore, at 7 days, more NGF- and BDNF-immunoreactive neurons were observed in the ventral horn of the spinal cord of rats that had received Panax notoginseng saponins than in those that received saline. These results indicate that Panax notoginseng saponins caused an upregulation of NGF and BDNF in rats with spinal cord transection, and improved hindlimb motor function.

    Related Articles | Metrics
    Buyang Huanwu decoction up-regulates Notch1 gene expression in injured spinal cord
    Zhan-peng Guo, Mi-na Huang, An-qi Liu, Ya-jiang Yuan, Jian-bo Zhao, Xi-fan Mei
    2015, 10 (8):  1321-1323.  doi: 10.4103/1673-5374.162767
    Abstract ( 212 )   PDF (272KB) ( 497 )   Save

    Expression of genes in the Notch signaling pathway is altered in the injured spinal cord, which indicates that Notch participates in repair after spinal cord injury. Buyang Huanwu decoction, a traditional Chinese herbal preparation, can promote the growth of nerve cells and nerve fibers; however, it is unclear whether Buyang Huanwu decoction affects the Notch signaling pathway in injured spinal cord. In this study, a rat model was established by injuring the T10 spinal cord. At 2 days after injury, rats were intragastrically administered 2 mL of 0.8 g/mL Buyang Huanwu decoction daily until sacrifice. Real-time reverse transcription polymerase chain reaction analysis demonstrated that at 7, 14 and 28 days after injury, the expression of Notch1 was increased in the Buyang Huanwu decoction group compared with controls. These findings confirm that Buyang Huanwu decoction can promote the expression of Notch1 in rats with incomplete spinal cord injury, and may indicate a mechanism to promote the repair of spinal cord injury.

    Related Articles | Metrics
    Blockade of transient receptor potential cation channel subfamily V member 1 promotes regeneration after sciatic nerve injury
    Fei Ren, Hong Zhang, Chao Qi, Mei-ling Gao, Hong Wang, Xia-qing Li
    2015, 10 (8):  1324-1331.  doi: 10.4103/1673-5374.162770
    Abstract ( 282 )   PDF (2926KB) ( 880 )   Save

    The transient receptor potential cation channel subfamily V member 1 (TRPV1) provides the sensation of pain (nociception). However, it remains unknown whether TRPV1 is activated after peripheral nerve injury, or whether activation of TRPV1 affects neural regeneration. In the present study, we established rat models of unilateral sciatic nerve crush injury, with or without pretreatment with AMG517 (300 mg/kg), a TRPV1 antagonist, injected subcutaneously into the ipsilateral paw 60 minutes before injury. At 1 and 2 weeks after injury, we performed immunofluorescence staining of the sciatic nerve at the center of injury, at 0.3 cm proximal and distal to the injury site, and in the dorsal root ganglia. Our results showed that Wallerian degeneration occurred distal to the injury site, and neurite outgrowth and Schwann cell regeneration occurred proximal to the injury. The number of regenerating myelinated and unmyelinated nerve clusters was greater in the AMG517-pretreated rats than in the vehicle-treated group, most notably 2 weeks after injury. TRPV1 expression in the injured sciatic nerve and ipsilateral dorsal root ganglia was markedly greater than on the contralateral side. Pretreatment with AMG517 blocked this effect. These data indicate that TRPV1 is activated or overexpressed after sciatic nerve crush injury, and that blockade of TRPV1 may accelerate regeneration of the injured sciatic nerve.

    Related Articles | Metrics
    Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury
    Hao Zhao, Bao-lin Yang, Zeng-xu Liu, Qing Yu, Wen-jun Zhang, Keng Yuan, Hui-hong Zeng, Gao-chun Zhu, De-ming Liu, Qing Li
    2015, 10 (8):  1332-1337.  doi: 10.4103/1673-5374.162769
    Abstract ( 244 )   PDF (1479KB) ( 763 )   Save

    Olfactory bulb tissue transplantation inhibits P2X2/3 receptor-mediated neuropathic pain. However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear. In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury. We assessed mechanical nociception in the rat models 7 and 14 days after surgery by measuring paw withdrawal threshold, and examined P2X2/3 receptor expression in L4–5 dorsal root ganglia using immunohistochemistry. Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia. At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals. Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4–5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

    Related Articles | Metrics
    Axon regeneration impediment: the role of paired immunoglobulin-like receptor B
    Jing Liu, Yan Wang, Wei Fu
    2015, 10 (8):  1338-1342.  doi: 10.4103/1673-5374.162771
    Abstract ( 257 )   PDF (345KB) ( 631 )   Save

    Regenerative capacity is weak after central nervous system injury because of the absence of an enhancing microenvironment and presence of an inhibitory microenvironment for neuronal and axonal repair. In addition to the Nogo receptor (NgR), the paired immunoglobulin-like receptor B (PirB) is a recently discovered coreceptor of Nogo, myelin-associated glycoprotein, and myelin oligodendrocyte glycoprotein. Concurrent blocking of NgR and PirB almost completely eliminates the inhibitory effect of myelin-associated inhibitory molecules on axonal regeneration. PirB participates in a key pathological process of the nervous system, specifically axonal regeneration inhibition. PirB is an inhibitory receptor similar to NgR, but their effects are not identical. This study summarizes the structure, distribution, relationship with common nervous system diseases, and known mechanisms of PirB, and concludes that PirB is also distributed in cells of the immune and hematopoietic systems. Further investigations are needed to determine if immunomodulation and blood cell migration involve inhibition of axonal regeneration.

    Related Articles | Metrics
    Neuroprotective effects of monosialotetrahexosylganglioside
    Ai-ping Xi, Zhong-xin Xu, Feng-li Liu, Yan-li Xu
    2015, 10 (8):  1343-1344.  doi: 10.4103/1673-5374.162950
    Abstract ( 293 )   PDF (147KB) ( 612 )   Save
    Monosialotetrahexosylganglioside (GM1) is a type of glycosphingolipid containing sialic acid and closely related to cell-cell recognition, adhesion and signal conduction. GM1 is mainly composed of ceramide and oligosaccharide chains, and it is the only ganglioside that can permeate the blood-brain barrier. The present review analyzed the neuroprotective effects of GM1. With further, in-depth study, the molecular mechanisms underlying the neuroprotective effects of ganglioside can be clarified and provide a solid foundation for clinical research. Moreover, well-designed, randomized controlled trials will open up new prospects for the clinical use of ganglioside.
    Related Articles | Metrics