Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (9): 1471-1476.doi: 10.4103/1673-5374.165519

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Cerebrospinal fluid from rats given hypoxic preconditioning protects neurons from oxygen-glucose deprivation-induced injury

Yan-bo Zhang1, Zheng-dong Guo2, Mei-yi Li3, Si-jie Li4, Jing-zhong Niu1, Ming-feng Yang1, Xun-ming Ji4, Guo-wei Lv4   

  1. 1 Department of Neurology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China
    2 Department of Endocrinology, Affiliated Hospital of Taishan Medical University, Taian, Shandong Province, China
    3 Department of Neurology, Shandong Taishan Chronic Disease Hospital, Taian, Shandong Province, China
    4 Hypoxia Medical Institute, Xuanwu Hospital, Capital Medical University, Beijing, China
  • Received:2015-07-06 Online:2015-09-28 Published:2015-09-28
  • Contact: Yan-bo Zhang, M.D., bbnnbn@163.com.
  • Supported by:

    This study was supported by a grant from the National Science and Technology Support Program of China, No. 2013BAI07B01; and the Natural Science Foundation of Shandong Province in China, No. ZR2012HQ014, ZR2011HM044; a grant from the Open Research Project of Beijing Key Laboratory for Hypoxic Preconditioning and Translational Medicine, No. 2015DYSY02.

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Abstract:

Hypoxic preconditioning activates endogenous mechanisms that protect against cerebral ischemic and hypoxic injury. To better understand these protective mechanisms, adult rats were housed in a hypoxic environment (8% O2/92% N2) for 3 hours, and then in a normal oxygen environment for 12 hours. Their cerebrospinal fluid was obtained to culture cortical neurons from newborn rats for 1 day, and then the neurons were exposed to oxygen-glucose deprivation for 1.5 hours. The cerebrospinal fluid from rats subjected to hypoxic preconditioning reduced oxygen-glucose deprivation-induced injury, increased survival rate, upregulated Bcl-2 expression and downregulated Bax expression in the cultured cortical neurons, compared with control. These results indicate that cerebrospinal fluid from rats given hypoxic preconditioning protects against oxygen-glucose deprivation-induced injury by affecting apoptosis-related protein expression in neurons from newborn rats.

Key words: nerve regeneration, hypoxic preconditioning, cerebrospinal fluid, cerebral cortex, oxygen- glucose deprivation, neurons, apoptosis, Bcl-2/Bax, neural regeneration