An effective inducer of dopaminergic neuron-like differentiation

doi:10.3969/j.issn.1673-5374.2013.05.006

Abstract

Abstract:

Rat bone marrow-derived mesenchymal stem cells were cultured and passaged in vitro. After induction with basic fibroblast growth factor for 24 hours, passage 3 bone marrow-derived mesenchymal stem cells were additionally induced into dopaminergic neurons using three different combinations with basic fibroblast growth factor as follows: 20% Xiangdan injection; all-trans retinoic acid + glial-derived neurotrophic factor; or sonic hedgehog + fibroblast growth factor 8. Results suggest that the bone marrow-derived mesenchymal stem cells showed typical neuronal morphological characteristics after induction. In particular, after treatment with sonic hedgehog + fibroblast growth factor 8, the expressions of nestin, neuron-specific enolase, microtubule- associated protein 2, tyrosine hydroxylase and vesicular monoamine transporter-2 in cells were significantly increased. Moreover, the levels of catecholamines in the culture supernatant were significantly increased. These findings indicate that Xiangdan injection, all-trans retinoic acid + glial-derived neurotrophic factor, and sonic hedgehog + fibroblast growth factor 8 can all induce dopaminergic neuronal differentiation from bone marrow-derived mesenchymal stem cells. In particular, the efficiency of sonic hedgehog + fibroblast growth factor 8 was highest.

Key words: neural regeneration, stem cells, mesenchymal stem cells, dopaminergic neuron, induction, differentiation, Xiangdan injection, all-trans retinoic acid, sonic hedgehog, fibroblast growth factor 8, catecholamine, grants-supported paper, photographs-containing paper, neuroregeneration

Wenyu Fu, Cui Lv, Wenxin Zhuang, Dandan Chen, E Lv, Fengjie Li, Xiaocui Wang. An effective inducer of dopaminergic neuron-like differentiation[J]. Neural Regeneration Research, 2013, 8(5): 427-434.

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