Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (14): 1101-1105.

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Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells

Yue Tang1, Yongchun Cui1, Fuliang Luo1, Xiaopeng Liu1, Xiaojuan Wang2, Aili Wu1, Junwei Zhao1, Zhong Tian1, Like Wu2   

  1. 1 State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China
    2 Beijing Wu Stem Cells Medical Center, Beijing 100070, China
  • Received:2011-11-01 Revised:2012-02-24 Online:2012-05-15 Published:2012-05-15
  • Contact: Like Wu, M.D., Beijing Wu Stem Cells Medical Center, Beijing 100070, China; Yue Tang, State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China likewu66@vip.sina.com tangyue1226@vip.sina.com
  • About author:Yue Tang☆, M.D., State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China Yongchun Cui and Yue Tang equally contributed to this work.

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Abstract:

In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson’s Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify whether the mechanism of action relates to abnormal α-synuclein accumulation in cells. Results showed that co-culture with bone marrow-derived mesenchymal stem cells enhanced PC12 cell viability and dopamine secretion in a cell dose-dependent manner. MitoLight staining was used to confirm that PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells demonstrate reduced levels of cell apoptosis. Immunocytochemistry and western blot analysis found the quantity of α-synuclein accumulation was significantly reduced in PC12 cell and bone marrow-derived mesenchymal stem cell co-cultures. These results indicate that bone marrow-derived mesenchymal stem cells can attenuate 6-hydroxydopamine-induced cytotoxicity by reducing abnormal α-synuclein accumulation in PC12 cells.

Key words: bone marrow-derived mesenchymal stem cells, α-synuclein, 6-hydroxydopamine, PC12 cells, dopamine, cell apoptosis, neurotoxicity, neural regeneration