Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (2): 156-161.doi: 10.3969/j.issn.1673-5374.2013.02.008

Previous Articles     Next Articles

Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system

Zhaoyang Li1, Bing Du1, Shengyang Li1, 2, Xiangchuan Lv1, Shenglai Zhou1, Yang Yu1, Wei Wang1, Zhihong Zheng1   

  1. 1 Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China
    2 College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, China
  • Received:2012-05-12 Revised:2012-07-03 Online:2013-01-15 Published:2013-01-15
  • Contact: Zhihong Zheng, M.D., Professor, Key Laboratory of Transgenetic Animal Research, Department of Laboratory Animal, China Medical University, Shenyang 110001, Liaoning Province, China, zhihongzheng@163.com.
  • About author:Zhaoyang Li★, Master, Lecturer.
  • Supported by:

    This study was supported by grants from the National Natural Science Foundation of China, No. 30770818; a grant from Education Department of Liaoning Province, No. 2009s109.

Abstract:

Apolipoprotein C2 is an important member of the apolipoprotein C family, and is a potent activator of lipoprotein lipase. In the central nervous system, apolipoprotein C2 plays an important role in the catabolism of triglyceride-rich lipoproteins. Studies into the exact regulatory mechanism of mouse apolipoprotein C2 expression have not been reported. In this study, seven luciferase expression vectors, which contained potential mouse apolipoprotein C2 gene promoters, were constructed and co-transfected with pRL-TK into HEK293T cells to investigate apolipoprotein C2 promoter activity. Luciferase assays indicated that the apolipoprotein C2 promoter region was mainly located in the +104 bp to +470 bp region. The activity of the different lengths of apolipoprotein C2 promoter region varied. This staggered negative-positive-negative arrangement indicates the complex regulation of apolipoprotein C2 expression and provides important clues for elucidating the regulatory mechanism of apolipoprotein C2 gene transcription.

Key words: neural regeneration, basic research, apolipoprotein C2, promoter, dual-luciferase reporter assay, transcriptional activity, regulatory elements, grants-supported paper, neuroregeneration