Neural Regeneration Research ›› 2021, Vol. 16 ›› Issue (4): 714-720.doi: 10.4103/1673-5374.295342

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Caveolin-1 downregulation promotes the dopaminergic neuron-like differentiation of human adipose-derived mesenchymal stem cells

Chao Han1, 2, #, Ya-Jun Wang3, #, Ya-Chen Wang1, 2, Xin Guan1, 4, Liang Wang1, 4, Li-Ming Shen1, 4, Wei Zou3, Jing Liu1, 2, 4, *#br#   

  1. 1 Stem Cell Clinical Research Center, Regenerative Medicine Center, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China;  2 National Joint Engineering Laboratory, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China;  3 College of Life Science, Liaoning Normal University, Dalian, Liaoning Province, China;  4 Dalian Innovation Institute of Stem Cell and Precision Medicine, Dalian, Liaoning Province, China
  • Online:2021-04-15 Published:2020-12-21
  • Contact: Jing Liu, PhD, liujing.dlrmc@hotmail.com.
  • Supported by:
    This work was supported by National Stem Cell Clinical Research Registered Project, No. CMR-20161129-1003 (to JL); Dalian Science and Technology Innovation Fund, No. 2018J11CY025 (to JL).

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Abstract: Previous studies have shown that caveolin-1 is involved in regulating the differentiation of mesenchymal stem cells. However, its role in the differentiation of human adipose mesenchymal stem cells into dopaminergic neurons remains unclear. The aim of this study was to investigate whether caveolin-1 regulates the differentiation of human adipose mesenchymal stem cells into dopaminergic-like neurons. We also examined whether the expression of caveolin-1 could be modulated by RNA interference technology to promote the differentiation of human adipose mesenchymal stem cells into dopaminergic-like neurons. The differentiation of human adipose mesenchymal stem cells into dopaminergic neurons was evaluated morphologically and by examining expression of the markers tyrosine hydroxylase, Lmx1a and Nurr1. The analyses revealed that during the differentiation of human adipose mesenchymal stem cells into dopaminergic neurons, the expression of caveolin-1 is decreased. Notably, the downregulation of caveolin-1 promoted the differentiation of human adipose mesenchymal stem cells into dopaminergic-like neurons, and it increased the expression of tyrosine hydroxylase, Lmx1a and Nurr1. Together, our findings suggest that caveolin-1 plays a negative regulatory role in the differentiation of dopaminergic-like neurons from stem cells, and it may therefore be a potential molecular target for strategies for regulating the differentiation of these cells. This study was approved by the Medical Ethics Committee of the First Affiliated Hospital of Dalian Medical University of China (approval No. PJ-KS-KY-2020-54) on March 7, 2017.

Key words: cells, factor, in vitro, neural differentiation, Parkinson’s disease, plasticity, protein, stem cells