Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (6): 405-412.

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Protective effects of curcumin in APPswe transfected SH-SY5Y cells

Wenke Yin1, Xiong Zhang2, 3, Yu Li2, 3   

  1. 1 Department of Pathology, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
    2 Department of Pathology, Chongqing Medical University, Chongqing 400016, China
    3 Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China
  • Received:2011-09-24 Revised:2011-12-22 Online:2012-02-25 Published:2012-02-25
  • Contact: Wenke Yin★, Master, Assistant, Department of Pathology, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China
  • About author:Wenke Yin★, Master, Assistant, Department of Pathology, North Sichuan Medical College, Nanchong 637000, Sichuan Province, China

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Abstract:

The APPswe plasmid was transfected into the neuroblastoma cell line SH-SY5Y to establish a cell model of Alzheimer’s disease. Graded concentration and time course experiments demonstrate that curcumin significantly upregulates phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor E2-related factor-2 (Nrf2), heme oxygenase 1 and ferritin expression, and that it significantly downregulates heme oxygenase 2, reactive oxygen species and amyloid-beta 40/42 expression. These effects of curcumin on PI3K, Akt and Nrf2 were blocked by LY294002 (PI3k inhibitor) and NF-E2-related factor-2 siRNA. The results indicate that the cytoprotection conferred by curcumin on APPswe transfected SH-SY5Y cells is mediated by its ability to regulate the balance between heme oxygenase 1 and 2 via the PI3K/Akt/Nrf2 intracellular signaling pathway.

Key words: Alzheimer’s disease, curcumin, phosphatidylinositol 3-kinase signaling pathway, heme oxygenase-1, heme oxygenase-2, neural regeneration