Neural Regeneration Research ›› 2012, Vol. 7 ›› Issue (36): 2867-2873.

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Protective effects of Lingguizhugan decoction on amyloid-beta peptide (25–35)-induced cell injury Anti-inflammatory effects

Feifei Xi1, Feng Sang2, Chunxiang Zhou1, 3, Yun Ling1   

  1. 1 Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China
    2 Henan Clinical Research Center of AIDS Controlled with Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003, Henan Province, China
    3 Nanjing BenQ Hospital, Nanjing 210036, Jiangsu Province, China
  • Received:2012-07-11 Revised:2012-10-10 Online:2012-12-25 Published:2012-12-25
  • Contact: Chunxiang Zhou, Ph.D.,Professor, Doctoral supervisor, Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province,China; Nanjing BenQ Hospital, Nanjing 210036,Jiangsu Province, China Chunxiangzhou@hotmail.com
  • About author:Feifei Xi☆, Studying for doctorate, Basic Medicine College, Nanjing University of Chinese Medicine, Nanjing 210046,Jiangsu Province,China

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Abstract:

In the present study, a human neuroblastoma cell line (SH-SY5Y) and BV-2 microglia were treated with amyloid-βpeptide (25–35), as a model of Alzheimer’s disease, to evaluate the protective effects of 10-3–10-8 g/mL Lingguizhugan decoction and to examine the underlying anti-inflammatory mechanism. Lingguizhugan decoction significantly enhanced the viability of SH-SY5Y cells with amyloid-β peptide-induced injury, and lowered levels of interleukin-1β,interleukin-6, tumor necrosis factor-α and nitric oxide in the culture supernatant of activated BV-2 microglia.The effects of 10-3 g/mL Lingguizhugan decoction were more significant. These results suggest that Lingguizhugan decoction can protect SH-SY5Y cells against amyloid-β peptide (25–35)-induced injury in a dose-dependent manner by inhibiting overexpression of inflammatory factors by activated microglia.

Key words: Alzheimer’s disease, Lingguizhugan decoction, phlegm-warming and fluid-dispersing, inflammatory reaction, SH-SY5Y, BV-2