Neuronal-like differentiation of bone marrow-derived mesenchymal stem cells induced by striatal extracts from a rat model of Parkinson’s disease

doi:10.3969/j.issn.1673-5374.2012.34.004

Abstract

Abstract:

A rat model of Parkinson’s disease was established by 6-hydroxydopamine injection into the medial forebrain bundle. Bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the femur and tibia, and were co-cultured with 10% and 60% lesioned or intact striatal extracts. The results showed that when exposed to lesioned striatal extracts, BMSCs developed bipolar or multi-polar morphologies, and there was an increase in the percentage of cells that expressed glial fibrillary acidic protein (GFAP), nestin and neuron-specific enolase (NSE). Moreover, the percentage of NSE-positive cells increased with increasing concentrations of lesioned striatal extracts. However, intact striatal extracts only increased the percentage of GFAP-positive cells. The findings suggest that striatal extracts from Parkinson’s disease rats induce BMSCs to differentiate into neuronal-like cells in vitro.

Key words: bone marrow-derived mesenchymal stem cell, Parkinson’s disease, striatal extract, induced differentiation, nerve cell, glial fibrillary acidic protein, nestin, neuron-specific enolase, neural stem cell, regeneration, neural regeneration

Xiaoling Qin, Wang Han, Zhigang Yu. Neuronal-like differentiation of bone marrow-derived mesenchymal stem cells induced by striatal extracts from a rat model of Parkinson’s disease[J]. Neural Regeneration Research, 2012, 7(34): 2673-2680.

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