Neural Regeneration Research ›› 2014, Vol. 9 ›› Issue (10): 1063-1067.doi: 10.4103/1673-5374.133172

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Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro

Xin Chen  1, Yongjie Zhu  1, Lulu Han  2, Hongting Lu  1, Xiwei Hao 1, Qian Dong 1   

  1. 1 Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
    2 Operating Room, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
  • Received:2014-04-05 Online:2014-05-26 Published:2014-05-26
  • Contact: Qian Dong, Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China, dong.qian@sohu.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81272986 and the Natural Science Foundation of Shandong Province, No. ZR2011HZ002.

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Abstract:

This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was significantly suppressed in transfected cells by all three sequence-specific siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was significantly decreased following transfection with CXCR4-specific siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.

Key words: nerve regeneration, chemokine receptor 4, small interfering RNA, neuroblastoma, invasion, Transwell chamber, liposome, NSFC grant, neural regeneration