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Treatment with β-sitosterol ameliorates the effects of cerebral ischemia/reperfusion injury by suppressing cholesterol overload, endoplasmic reticulum stress, and apoptosis

• Figure 3｜β-Sitosterol reduces the neuronal apoptosis caused by MCAO in mice.

  

  
  结果：To determine whether β-sitosterol decreases neuronal apoptosis, we performed Nissl and TUNEL staining of post-infarct brain sections (Figure 3). Nissl staining showed significant morphological changes in the peri-infarct zone in MCAO mice, including neuronal cell loss, neuronal nucleus shrinkage, cell swelling, and interstitial edema (Figure 3A). The degree of these morphological changes was significantly lower in the β-sitosterol-treated mice than in the model group mice (one-way ANOVA, F(2, 15) = 93.02, P < 0.0001; Figure 3A). Similar results were obtained by TUNEL staining. As shown in Figure 3B, no apoptotic cells were observed in the brain tissue of mice in the sham group. In contrast, many apoptotic cells were observed in the brain tissue of mice in the model group. In comparison with the model group, fewer apoptotic neurons were observed in the groups treated with β-sitosterol (one-way ANOVA, F(2, 15) = 43.09, P < 0.0001; Figure 3B). These findings indicated that β-sitosterol has an anti-apoptotic effect after MCAO.
  
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发布日期： 2023-09-02  浏览： 79