中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (28): 2221-2226.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

缺氧缺血性脑病新生大鼠松果体clock基因的表达

  

  • 收稿日期:2012-01-09 修回日期:2012-04-23 出版日期:2012-10-05 发布日期:2012-10-05

Expression of Clock genes in the pineal glands of newborn rats with hypoxic-ischemic encephalopathy

Bin Sun1, Xing Feng1, Xin Ding1, Li Bao2, Yongfu Li3, Jun He4, Meifang Jin1   

  1. 1 Division of Neonatology, Affiliated Children’s Hospital of Soochow University, Suzhou 215003, Jiangsu Province, China
    2 Department of General Pediatrics, Yixing People’s Hospital, Yixing 214200, Jiangsu Province, China
    3 Division of Neonatology, Suzhou Municipal Hospital (Main Campus), Suzhou 215002, Jiangsu Province, China
    4 Jiangsu Institute of Hematology and Blood Diseases, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • Received:2012-01-09 Revised:2012-04-23 Online:2012-10-05 Published:2012-10-05
  • Contact: Xing Feng, Ph.D., Professor, Division of Neonatology, Affiliated Children’s Hospital of Soochow University, Suzhou 215003, Jiangsu Province, China xing_feng66@hotmail.com
  • About author:Bin Sun☆, Ph.D., Associate professor, Division of Neonatology, Affiliated Children’s Hospital of Soochow University, Suzhou 215003, Jiangsu Province, China

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Abstract:

Clock genes are involved in circadian rhythm regulation, and surviving newborns with hypoxic-ischemic encephalopathy may present with sleep-wake cycle reversal. This study aimed to determine the expression of the clock genes Clock and Bmal1, in the pineal gland of rats with hypoxic-ischemic brain damage. Results showed that levels of Clock mRNA were not significantly changed within 48 hours after cerebral hypoxia and ischemia. Expression levels of CLOCK and BMAL1 protein were significantly higher after 48 hours. The levels of Bmal1 mRNA reached a peak at 36 hours, but were significantly reduced at 48 hours. Experimental findings indicate that Clock and Bmal1 genes were indeed expressed in the pineal glands of neonatal rats. At the initial stage (within 36 hours) of hypoxic-ischemic brain damage, only slight changes in the expression levels of these two genes were detected, followed by significant changes at 36-48 hours. These changes may be associated with circadian rhythm disorder induced by hypoxic-ischemic brain damage.

Key words: brain hypoxia, cerebral ischemia, neonatal rats, pineal gland, Clock, Bmal1, mRNA, protein, brain, neural regeneration