中国神经再生研究(英文版)

中国神经再生研究(英文版)

• 观点:神经损伤修复保护与再生 •    下一篇

Bumetanide promotes nerve regeneration and recovery of cognitive function in the dentate gyrus of the rat hippocampus after cerebral ischemia

  

  • 出版日期:2015-01-01 发布日期:2015-01-01

Bumetanide promotes nerve regeneration and recovery of cognitive function in the dentate gyrus of the rat hippocampus after cerebral ischemia

Wangshu Xu 1, Xuan Sun 2, Chengguang Song 1, 3, Xiaopeng Mu 1 and Chuansheng Zhao 1   

  1. 1Department of Neurology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning Province, PR China
    2Department of Interventional Neuroradiology, Beijing Tian Tan Hospital, Capital Medical University, Beijing, PR China
    3Department of Neurology, Benxi Central Hospital of China Medical University, Benxi, Liaoning Province, PR China
  • Online:2015-01-01 Published:2015-01-01
  • Contact: Chuansheng Zhao, No.155, North Nanjing Street, Heping District, Shenyang 110001, Liaoning Province, PR China. Phone: 86-24-83283026; Fax: 86-24-83282315; Email: xws921@sina.com, cszhao@mail.cmu.edu.cn.
  • Supported by:

    Wangshu Xu and Xuan Sun contributed equally to this work.

Abstract:

Bumetanide is a widely used diuretic in the clinic. In the acute stage of cerebral ischemia, bumetanide can reduce brain edema through the blood-brain barrier, decrease infarct size, and has a protective effect on the brain. However, no relevant studies concerned neuroprotection and nerve regeneration in the chronic stage of cerebral ischemia. This study sought to explore the effects of bumetanide on neurogenesis in the dentate gyrus of the rat hippocampus after the acute stage of cerebral ischemia. Adult Wistar rats weighing 200–250 g were randomly divided into four groups: (1) sham operation group (SHAM group), (2) sham operation + bumetanide group (SHAM + BUM group), (3) ischemia group (ISC group), and (4) ischemia + bumetanide group (ISC + BUM group). Models of cerebral ischemia were established by intracranial injection of endothelin-1. 1 week later, bumetanide was given in the skull using an osmotic pump for 3 weeks, 20 mg/kg per day. Morris water maze was used to assess learning and memory abilities in rats. Immumofluorescence method was utilized to observe DCX-positive cells in the dentate gyrus of the rat hippocampus. By confocal microscopy, series layer in the direction of Z-axis of DCX-positive cells in the dentate gyrus was obtained, and three-dimensional reconstruction was carried out in newborn dendrites and axons. NIH ImageJ software was then applied to track and measure dendrites, axons and their branches. Bumetanide promoted regeneration of neural stem cells and dendrite development in the hippocampal dentate gyrus after cerebral ischemia.


Wangshu Xu and Xuan Sun contributed equally to this work.

Key words: cerebral ischemia, bumetanide, neurogenesis, NKCCl, functional recovery