中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (27): 2131-2135.

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

针刺上调猫备用背根神经节神经元血小板源性生长因子的表达

  

  • 收稿日期:2012-02-18 修回日期:2012-06-22 出版日期:2012-09-25 发布日期:2012-09-25

Electroacupuncture upregulated platelet derived growth factor expression in spared dorsal root ganglion of cats

Xifeng Wang1, Lianshuang Zhang2, Xiaobo Xu3, Wei Zhao2, Guixiang Liu2   

  1. 1 Intensive Care Unit of Yantai Yuhuangding Hospital, Yantai 264000, Shandong Province, China 2 Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, Shandong Province, China 3 Neuroscience Department, University of Southern California, CA 90007, USA
  • Received:2012-02-18 Revised:2012-06-22 Online:2012-09-25 Published:2012-09-25
  • Contact: Lianshuang Zhang, Master, Associate professor, Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, Shandong Province, China zls197600@126.com
  • About author:Xifeng Wang★, Master, Attending physician, Intensive Care Unit of Yantai Yuhuangding Hospital, Yantai 264000, Shandong Province, China

PDF

825

Abstract:

A bilateral spared dorsal root ganglion model was established in healthy adult cats by bilateral resection of L1-5 and L7–S2 dorsal root ganglia. L6 dorsal root ganglia were spared. Zusanli (ST36) and Xuanzhong (BL39) or Futu (ST32) and Sanyinjiao (SP6) were alternatively electro-stimulated on the right leg. Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7th day following injury. After 7 days of acupuncture, the total number of positive and large neurons staining for platelet-derived growth factor on the acupuncture side significantly increased compared to the non-acupuncture side. After acupuncture for 14 days, the total positive and medium-small sized neurons significantly increased compared with the non-acupuncture side. Results indicate that acupuncture promoted the synthesis of platelet-derived growth factor in spared dorsal root ganglia.

Key words: platelet-derived growth factor, acupuncture, dorsal root ganglion, immunohistochemistry, cat, traditional Chinese medicine, neural morphology, neural regeneration