中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (21): 1605-1610.

• 原著:脑损伤修复保护与再生 •    下一篇

Buyang Huanwu decoction enhances cell membrane fluidity in rats with cerebral ischemia/reperfusion

  

  • 收稿日期:2012-01-18 修回日期:2012-06-16 出版日期:2012-07-25 发布日期:2012-07-25

Buyang Huanwu decoction enhances cell membrane fluidity in rats with cerebral ischemia/reperfusion

Chenxu Li   

  1. Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China
  • Received:2012-01-18 Revised:2012-06-16 Online:2012-07-25 Published:2012-07-25
  • Contact: Chenxu Li, Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China lichenxv20021208@yahoo. com.cn
  • About author:Chenxu Li☆, M.D., Associate professor, Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China

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Abstract:

After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats. Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements. Brain nerve cells from rats with ischemia/reperfusion injury were cultured with 1-100 mg/mL Buyang Huanwu decoction. Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH˙) and superoxide (O2˙) free radicals dose-dependently. These findings suggest that Buyang Huanwu decoction can protect against cell membrane fluidity changes in rats with ischemia/ reperfusion injury by scavenging free radicals.

Key words: Buyang Huanwu decoction, ischemia/reperfusion, electron spin resonance, cell membrane fluidity, free radical, brain, neural regeneration