中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (6): 1199-1200.doi: 10.4103/1673-5374.386403

• 观点:周围神经损伤修复保护与再生 • 上一篇    下一篇

结肠类器官背根神经节神经元共培养研究内脏疼痛中上皮神经元信号传导的作用

  

  • 出版日期:2024-06-15 发布日期:2023-11-17

Investigating epithelial-neuronal signaling contribution in visceral pain through colon organoid-dorsal root ganglion neuron co-cultures

Francesco Margiotta,  Lorenzo Di Cesare Mannelli,  Antonino Morabito, Carla Ghelardini, Elena Lucarini*#br#   

  1. Department of Neuroscience, Psychology, Drug Research and Child Health - NEUROFARBA - Pharmacology and Toxicology Section, University of Florence, Florence, Italy (Margiotta F, Di Cesare Mannelli L, Ghelardini C, Lucarini E)
    Department of Neuroscience, Psychology, Drug Research and Child Health - NEUROFARBA, University of Florence, Florence, Italy (Morabito A)
    Meyer Children’s Hospital IRCCS, Florence, Italy (Morabito A)
  • Online:2024-06-15 Published:2023-11-17
  • Contact: Elena Lucarini, PhD, elena.lucarini@unifi.it.
  • Supported by:
    This work was supported by the co-funding European Union - Next Generation EU, in the context of The National Recovery and Resilience Plan, Investment 1.5 Ecosystems of Innovation, Project Tuscany Health Ecosystem (THE), CUP: B83C22003920001 and Italian Ministry of University and Research (MIUR) - Dipartimenti di Eccellenza 2023-2027 to the Department NEUROFARBA (to CG).

摘要: https://orcid.org/0000-0002-3459-2660 (Elena Lucarini)

Abstract: Abdominal pain is a common symptom associated with irritable bowel syndrome and inflammatory bowel diseases (IBDs), affecting about 20% of the global population (Grundy et al., 2019). Current pain therapies are poorly effective on visceral pain of intestinal origin and present several side effects, hence the need to identify novel molecular and cellular targets for drug development. The pathophysiology of visceral-abdominal pain, which often originates from the colorectal region, involves several actors, including the gut microbiome, intestinal epithelium, immune system, and nervous system at different levels through the gut-brain axis (Lucarini et al., 2020, 2022; Najjar et al., 2020). Nociceptive stimuli from the bowel to the central nervous system are mainly encoded by spinal afferents, whose cell bodies reside within the thoracolumbar and lumbosacral dorsal root ganglia (DRGs; Grundy et al., 2019). Visceral pain results from an altered neuronal transduction and transmission of stimuli generated within the gut. Current evidence attests that visceral hypersensitivity is a complex phenomenon, consisting of multiple mechanisms, with immune cells playing a role in hypersensitizing colon afferents through the release of different mediators (Grundy et al., 2019). However, pain is also reported without any inflammatory status, suggesting factors other than the inflammatory/immune signaling in driving pain transmission/persistence.