中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (17): 1285-1982.

• 原著:周围神经损伤修复保护与再生 •    下一篇

Gene expression profiling of the rat sciatic nerve in early Wallerian degeneration after injury

  

  • 收稿日期:2012-02-10 修回日期:2012-04-23 出版日期:2012-06-15 发布日期:2012-06-15

Gene expression profiling of the rat sciatic nerve in early Wallerian degeneration after injury

Dengbing Yao1, 2, Meiyuan Li1, Dingding Shen1, Fei Ding, Shibi Lu3, Qin Zhao3, Xiaosong Gu1   

  1. 1  Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226019, Jiangsu Province, China
    2  School of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China
    3  Key Laboratory of People’s Liberation Army, Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2012-02-10 Revised:2012-04-23 Online:2012-06-15 Published:2012-06-15
  • Contact: Qin Zhao, Ph.D., Professor, Key Laboratory of People’s Liberation Army, Institute of Orthopaedics, Chinese PLA General Hospital, Beijing 100853, China; Xiaosong Gu, Ph.D., Professor, Doctorial supervisor, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226019, Jiangsu Province, China drzhaoqing@sina.com;neurongu@public.nt.js.cn
  • About author:Dengbing Yao☆, Ph.D., Jiangsu Key Laboratory of Neuroregeneration, School of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China Dengbing Yao and Meiyuan Li contributed equally to this work.

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Abstract:

Wallerian degeneration is an important area of research in modern neuroscience. A large number of genes are differentially regulated in the various stages of Wallerian degeneration, especially during the early response. In this study, we analyzed gene expression in early Wallerian degeneration of the distal nerve stump at 0, 0.5, 1, 6, 12 and 24 hours after rat sciatic nerve injury using gene chip microarrays. We screened for differentially-expressed genes and gene expression patterns. We examined the data for Gene Ontology, and explored the Kyoto Encyclopedia of Genes and Genomes Pathway. This allowed us to identify key regulatory factors and recurrent network motifs. We identified 1 546 differentially-expressed genes and 21 distinct patterns of gene expression in early Wallerian degeneration, and an enrichment of genes associated with the immune response, acute inflammation, apoptosis, cell adhesion, ion transport and the extracellular matrix. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT, ErbB, transforming growth factor-β, T cell receptor and calcium signaling pathways. Key factors included interleukin-6, interleukin-1, integrin, c-sarcoma, carcinoembryonic antigen-related cell adhesion molecules, chemokine (C-C motif) ligand, matrix metalloproteinase, BH3 interacting domain death agonist, baculoviral IAP repeat-containing 3 and Rac. The data were validated with real-time quantitative PCR. This study provides a global view of gene expression profiles in early Wallerian degeneration of the rat sciatic nerve. Our findings provide insight into the molecular mechanisms underlying early Wallerian degeneration, and the regulation of nerve degeneration and regeneration.

Key words: Wallerian degeneration, sciatic nerve, microarrays, expression profiling, rats, neural regeneration