中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (17): 1338-1342.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury

  

  • 收稿日期:2011-12-15 修回日期:2012-03-06 出版日期:2012-06-15 发布日期:2012-06-15

Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury

Junliang Li1, Xinke Xu1, Shanyi Zhang1, Meiguang Zheng1, Zhonghua Wu2, Yinlun Weng1, Leping Ouyang1, Jian Yu1, Fangcheng Li1   

  1. 1  Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China
    2  Department of Neurosurgery, Henan Provincial People’s Hospital, Zhengzhou 450003, Henan Province, China
  • Received:2011-12-15 Revised:2012-03-06 Online:2012-06-15 Published:2012-06-15
  • Contact: Fangcheng Li, M.D., Chief physician, Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China sjwklfc@yahoo.com.cn
  • About author:Junliang Li★, Master, Attending physician, Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China

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Abstract:

In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.

Key words: glucose transporter 3, ischemia, glucose uptake rate, apoptosis, nuclear factor kappaB