中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (16): 1205-1212.

• 原著:神经损伤修复保护与再生 •    下一篇

Autophagy and apoptosis during adult adipose- derived stromal cells differentiation into neuron-like cells in vitro

  

  • 收稿日期:2012-02-20 修回日期:2012-05-03 出版日期:2012-06-05 发布日期:2012-06-05

Autophagy and apoptosis during adult adipose- derived stromal cells differentiation into neuron-like cells in vitro

Yanhui Lu1, Xiaodong Yuan1, Ya Ou1, Yanan Cai1, Shujuan Wang1, Qiaoyu Sun1, Wenli Zhang2   

  1. 1  Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China
    2  Hebei Union University, Tangshan 063000, Hebei Province, China
  • Received:2012-02-20 Revised:2012-05-03 Online:2012-06-05 Published:2012-06-05
  • Contact: Xiaodong Yuan, Professor, Chief physician, Master’s supervisor, Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China yxd68@sohu.com
  • About author:Yanhui Lu★, Studying for master’s degree, Department of Neurology, Kailuan General Hospital, Hebei Union University, Tangshan 063000, Hebei Province, China

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Abstract:

β-mercaptoethanol can induce adult adipose-derived stromal cells to rapidly and efficiently differentiate into typical neuron-like cells in vitro. Immunohistochemistry showed that neuron specific enolase and neurofilament-200 expression gradually increased with the extension of induction time, and peaked at 5 hours. By contrast, glial fibrillary acidic protein was negatively expressed at all time points. Induced cells possessed a typical Nissl body, apoptosis showing condensed chromatin in the nucleus, autophagosomes with a bilayered membrane and autolysosomes in the cytoplasm at 5 hours. TUNEL assay and immunohistochemistry and immunofluorescence demonstrated that apoptosis and caspase-3 expression increased and peaked at 8 hours. Immunohistochemistry and immunofluorescence showed that microtubule- associated protein light chain 3 gradually increased with induction and reached a peak at 5 hours. These results indicate that autophagy played an important role in protecting cells during adult adipose-derived stromal cells differentiation into neuron-like cells in vitro.

Key words: adult adipose-derived stromal cells, neuron-like cells, differentiation, apoptosis, autophagy, neural regeneration