中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (15): 1171-1178.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation

  

  • 收稿日期:2012-01-21 修回日期:2012-04-19 出版日期:2012-05-25 发布日期:2012-05-25

Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation

Aijun Hu1, Baoqi Zuo2, 3, Feng Zhang4, Qing Lan5, Huanxiang Zhang4   

  1. 1 Department of Otolaryngy, the Second Affiliated Hospital of Soochow University, Suzhou 215021, Jiangsu Province, China
    2 College of Textiles and Clothing Engineering, Soochow University, Suzhou 215021, Jiangsu Province, China
    3 National Engineering Laboratory for Modern Silk, Suzhou 215023, Jiangsu Province, China
    4 Jiangsu Province Key Laboratory of Stem Cell Research, Medical College of Soochow University, Suzhou 215007, Jiangsu Province, China
    5 Department of Neurology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China
  • Received:2012-01-21 Revised:2012-04-19 Online:2012-05-25 Published:2012-05-25
  • Contact: Qing Lan, Department of Neurology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China; Huanxiang Zhang, Jiangsu Province Key Laboratory of Stem Cell Research, Medical College of Soochow University, Suzhou 215007, Jiangsu Province, China szlq006@yahoo.com.cn; hzhang@suda.edu.cn
  • About author:Aijun Hu, Department of Otolaryngy, the Second Affiliated Hospital of Soochow University, Suzhou 215021, Jiangsu Province, China

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Abstract:

In this study, Schwann cells, at a density of 1 × 105 cells/well, were cultured on regenerated silk fibroin nanofibers (305 ± 84 nm) prepared using the electrospinning method. Schwann cells cultured on the silk fibroin nanofibers appeared more ordered, their processes extended further, and they formed more extensive and complex interconnections. In addition, the silk fibroin nanofibers had no impact on the proliferation of Schwann cells or on the secretion of ciliary neurotrophic factor, brain-derived neurotrophic factor or nerve growth factor. These findings indicate that regenerated electrospun silk fibroin nanofibers can promote Schwann cell adhesion, growth and proliferation, and have excellent biocompatibility.

Key words: peripheral nerve regeneration, nerve tissue engineering, Schwann cells, silk fibroin,  , electrospinning, neural regeneration