中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (on line): 1-6.

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Pannexin 1 contributes to hypotonicity-induced ATP release in Schwann cells

  

  • 出版日期:2020-01-01 发布日期:2020-05-30

Zhong-Ya Wei1, Hui-Lin Qu1, Yu-Juan Dai2, Qian Wang1, Zhuo-Min Ling2, Wen-Feng Su1, Ya-Yu Zhao1, Wei-Xing Shen2, Gang Chen1, 2, 3   

  1. 1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China;  2Medical School of Nantong University, Nantong, Jiangsu Province, China;  3Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China
  • Online:2020-01-01 Published:2020-05-30
  • Contact: Zhong-Ya Wei, Ph.D., weizhongya08yan@126.com; Gang Chen, Ph.D., M.D., chengang6626@ntu.edu.cn.
  • Supported by:
    This study was supported by the National Key Research and Development Program of China, No. 2017YFA0104700; National Natural Science Foundation of China, Nos. 31900718, 31872773; Basic Research Program of the Education Department of Jiangsu Province of China, Nos. 19KJB180024, 18KJB180020; Postdoctoral Science Foundation of China, No. 2019M651925, Jiangsu Students’ Platform for Innovation and Entrepreneurship Training Program of China, No. 201810304031Z; Six Talent Peaks Project in Jiangsu Province of China, No. WSN-007.

摘要: orcid: 0000-0003-3669-5687 (Gang Chen)

Abstract: Pannexin 1 (Panx 1) is highly permeable to ATP and other signaling molecules. Previous studies have implicated the expression of Panx 1 in the nervous system, including astrocytes, microglia and neurons. However, the distributions and function of pannexins in the peripheral nervous system are not clear. In this study, according to the investigation of mRNA and protein levels in Panxs, we find that Panx 1 is highly expressed in Schwann cells compared with other pannexin subtypes. Using blockers and small interfering RNA-knockdown in cultured Schwann cells, we tested the hypothesis that Panx 1 contributes to hypotonic solution-induced ATP release and investigated mechanism regulating this activity. Hypotonic solution-induced ATP release was markedly attenuated by Panx 1 blockers or Panx 1-specific small interfering RNA. Moreover, hypotonicity-induced ATP release was dependent on Ras homolog family member A signaling and intact cytoskeleton. Our findings indicate that Panx 1 is important for ATP release in Schwann cells by regulating Ras homolog family member A and cytoskeleton arrangment.

Key words: peripheral nerve, injury, pannexin 1, ATP, Schwann cells, Ras homolog family member A, cytoskeleton