中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (on line): 1-7.

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Identification of potential oxidative stress biomarker in erythrocytes for spinal cord injury with mass spectrometry

  

  • 出版日期:2020-01-01 发布日期:2020-07-31

Li-Jian Zhang1, 2, 3, Yao Chen1, 2, 3, Lu-Xuan Wang1, Xiao-Qing Zhuang4, He-Chun Xia2, 3   

  1. 1School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 2Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 3Ningxia Human Stem Cell Research Institute, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China; 4Department of Nuclear Medicine, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, China
  • Online:2020-01-01 Published:2020-07-31
  • Contact: He-Chun Xia, MD, xhechun@nyfy.com.cn; Xiao-Qing Zhuang, PhD, zhuangxq@nyfy.com.cn.
  • Supported by:
    This work was supported by the Key Research Projects of the Ningxia Hui Autonomous Region of China, No. 2018BCG01002 (to HCX); and the Plan of Postgraduate Education Innovation, Discipline Construction Project of Ningxia, China (2017), No. YXW2017014 (to LJZ).

Abstract: Oxidative stress is a hallmark of secondary injury associated with spinal cord injury (SCI). Thus, stable and specific oxidative biomarkers of SCI need to be developed and validated. A lack of nuclei and mitochondria makes erythrocytes as valuable bioindicators, especially for oxidative stress. In this study, we examined the proteome dynamics of erythrocytes from Beagle dogs in the acute phase (0, 1, 3 days after SCI) and subacute phase (7, 14, 21 days after SCI) with mass spectrometry-based approaches. In total, 26 proteins were found to be differentially expressed at different time points following SCI. Bioinformatic analysis revealed that those differentially expressed proteins involved in oxidative stress pathway, including glutathione metabolism, lipid metabolism, and pentose phosphate pathways. The dysregulated expression of differentially expressed proteins like glutathione synthetase, transaldolase, and myeloperoxidase was validated by western blot analysis. This longitudinal study provides a comprehensive and quantitative picture of erythrocytes between acute and subacute stages of SCI dogs and suggests glutathione synthetase, transaldolase, and myeloperoxidase for further characterization as potential biomarkers to monitor oxidative stress in SCI. The study was approved by the Experimental Animal Centre of Ningxia Medical University (approval No. 2017-073) on February 13, 2017.

Key words: spinal cord injury, dog, acute phase, subacute phase, peripheral blood, erythrocytes, mass spectrometry, bioinformatic analysis, oxidative stress, biomarkers