中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (13): 965-970.

• 原著:神经损伤修复保护与再生 •    下一篇

Actin-binding Rho activating protein is expressed in the central nervous system of normal adult rats

  

  • 修回日期:2012-04-07 出版日期:2012-05-05 发布日期:2012-05-05

Actin-binding Rho activating protein is expressed in the central nervous system of normal adult rats

Lihua Liu1, 2, Mingying Luo1, Baolin Yang1, Xiaoqiong Wu3, Wu Zhu1, Yinglu Guan1, Weijun Cai1,Kerstin Troidl4, Wolfgang Schaper4, Jutta Schaper4   

  1. 1 Department of Histology & Embryology, School of Basic Medical Sciences, Central South University, Changsha 410013, Hunan Province,
    China
    2 Medical College, Hunan Normal University, Changsha 410013, Hunan Province, China
    3 Department of Anatomy & Neurobiology, School of Basic Medical Sciences, Central South University, Changsha 410013, Hunan Province,
    China
    4 Max-Planck-Institute for Heart and Lung Research, Arteriogenesis Research Group, Parkstr. 1, D-61231 Bad Nauheim, Germany
  • Revised:2012-04-07 Online:2012-05-05 Published:2012-05-05
  • Contact: Weijun Cai, M.D., Ph.D.,Professor, Department of Histology & Embryology,School of Basic Medical Sciences, Central South University, Changsha 410013, Hunan Province, China; Wolfgang Schaper, M.D., Ph.D., Professor, Max-Planck-Institute for Heart and Lung Research,Arteriogenesis Research Group, Parkstr. 1, D-61231 Bad Nauheim, Germany;Kerstin Troidl, Ph.D.,Professor, Max-Planck-Institute for Heart and Lung Research, Arteriogenesis Research Group, Parkstr. 1, D-61231 Bad Nauheim,Germany wjcai@yahoo.com;wolfgang.schaper@mpi-bn.mpg.de;kerstin.troidl@mpi-bn.mpg. de
  • About author:Lihua Liu , Master, Lecturer★ ,Department of Histology & Embryology, School of Basic Medical Sciences, Central South University, Changsha 410013, Hunan Province,China; Medical College,Hunan Normal University,Changsha 410013, Hunan Province, China Lihua Liu and Mingying Luo contributed equally to this work.

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Abstract:

Previous studies show that actin-binding Rho activating protein (Abra) is expressed in cardiomyocytes and vascular smooth muscle cells. In this study, we investigated the expression profile of Abra in the central nervous system of normal adult rats by confocal immunofluorescence. Results showed that Abra immunostaining was located in neuronal nuclei, cytoplasm and processes in the central nervous system, with the strongest staining in the nuclei; in the cerebral cortex, Abra positive neuronal bodies and processes were distributed in six cortical layers including molecular layer, external granular layer, external pyramidal layer, internal granular layer, internal pyramidal layer and polymorphic layer; in the hippocampus, the cell bodies of Abra positive neurons were distributed evenly in pyramidal layer and granular layer, with positive processes in molecular layer and orien layer; in the cerebellar cortex, Abra staining showed the positive neuronal cell bodies in Purkinje cell layer and granular layer and positive processes in molecular layer; in the spinal cord,Abra-immunopositive products covered the whole gray matter and white matter; co-localization studies showed that Abra was co-stained with F-actin in neuronal cytoplasm and processes, but weakly in the nuclei. In addition, in the hippocampus, Abra was co-stained with F-actin only in neuronal processes, but not in the cell body. This study for the first time presents a comprehensive overview of Abra expression in the central nervous system, providing insights for further investigating the role of Abra in the mature central nervous system.

Key words: actin-binding Rho activating protein, actin cytoskeleton, confocal immunofluorescence, striated muscle, nervous tissue, neural regeneration