中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (on line): 1-6.

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Neat1 decreases neuronal apoptosis after OGD by influencing pidd1-caspase-2 pathway

  

  • 出版日期:2020-01-01 发布日期:2020-12-25

Weina Chai, Yifan Wu, Zhimin Wu, Yanfeng Xie, Quanhong Shi, Wei Dan, Yan Zhan, Jianjun Zhong, Wei Tang, Xiaochuan Sun* , Li Jiang*   

  1. Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, China
  • Online:2020-01-01 Published:2020-12-25
  • Contact: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, China

Abstract: The purpose of the study was to explore the effects of Neat1 on neuronal apoptosis, and to investigate the possible mechanism by establishing OGD (Oxygen and Glucose Deprivation, OGD) model on neurons to simulate injury secondary to traumatic brain injury. Primary neurons were cultured, and then treated with OGD. Neat1 over-expression and downregulation adenovirus were used to infect primary neurons. Therefore, four groups were founded according to the levels of Neat1, namely the control group, OGD group, Neat1 over-expression group and Neat1 down-regulation group. The expression levels of PIDD1 (p53-induced protein with a death domain 1, PIDD1), caspase-2, cleaved caspase-3 and cytochrome C in each group were detected by Western-Blot. Moreover, the expression of cytochrome C in mitochondria of neurons in different groups was also detected. In addition, the cell viability and apoptosis rate of each group were detected by CCK-8 (cell-counting kit 8, CCK-8) and TUNEL (TdT-mediated dUTP Nick-End Labeling, TUNEL) respectively. The apoptosis rates of neurons were significantly reduced in Neat1 over-expression group, but significantly increased in Neat1 down-regulation group, as compared with the OGD group (p < 0.05). Similarly, the levels of PIDD1, caspase-2 and cleaved caspase-3 were significantly decreased in Neat1 over-expression group, and increased in Neat1 down-regulation group. Furthermore, in Neat1 over-expression group, neurons had significantly lower level of cytosolic cytochrome C but obviously higher level of mitochondrial cytochrome C as compared with Neat1 down-regulation group (p < 0.05). Up-regulation of Neat1 may decrease neuronal apoptosis after OGD by influencing PIDD1-caspase-2 pathway. 

Key words: LncRNA-Neat1, apoptosis, OGD, cytochrome C, caspase-2, caspase-3, PIDD1, mitochondria, neuron, traumatic brain injury