中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (7): 1331-1335.doi: 10.4103/1673-5374.301033

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

反映脊髓损伤后神经炎症过程的生物标志物

  


  • 出版日期:2021-07-15 发布日期:2021-01-07
  • 基金资助:

    韩国国家研究基金资助

Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury

Poornima D. E. Weerasinghe-Mudiyanselage1, 2, Jeongtae Kim1, 3, Yuna Choi1, Changjong Moon2, Taekyun Shin1, *, Meejung Ahn1, 4, *   

  1. 1 Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, Republic of Korea;  2 Department of Veterinary Anatomy, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Republic of Korea;  3 Department of Anatomy, Kosin University College of Medicine, Busan, Republic of Korea;  4 Department of Animal Science, College of Life Science, Sangji University, Wonju, Republic of Korea
  • Online:2021-07-15 Published:2021-01-07
  • Contact: Meejung Ahn, DVM, PhD, meeahn20@sangji.ac.kr; Taekyun Shin, DVM, PhD, shint@jejunu.ac.kr.
  • Supported by:
    MA was supported by the National Research Foundation of Korea (Grant No. NRF-2018R1D1A1B07050916).

摘要:

尽管研究已表明Ninjurin-1参与SCI后的细胞运输和轴突生长,但其确切作用仍不清楚。为评估Ninjurin-1在大鼠压迫性损伤脊髓组织(SCI)中的表达,以阐明该蛋白的参与炎症过程的作用。蛋白质印迹分析显示,与假对照组相比,脊髓损伤大鼠脊髓组织Ninjurin-1的水平直至损伤后第4天(PI)均呈上调状态,此后略有下降。免疫组织化学分析显示,与假手术对照相比,Ninjurin-1免疫反应在伤后第1和4天急剧增加,而在伤后7和21天免疫反应有所减弱。假手术对照组脊髓组织血管内皮细胞,室管膜细胞和一些神经胶质细胞中Ninjurin-1免疫染色较弱,而大鼠脊髓损伤处巨噬细胞/小胶质细胞和反应性星形胶质细胞Ninjurin-1免疫染色。说明多种细胞,包括血管内皮细胞,巨噬细胞/小胶质细胞和星形胶质细胞分泌Ninjurin-1,这些细胞参与了压迫性脊髓损伤的病理生理过程。

https://orcid.org/0000-0002-7302-9694 (Meejung Ahn); 

https://orcid.org/0000-0002-9851-4354 (Taekyun Shin)

Abstract: Previous studies have shown that Ninjurin-1 participates in cell trafficking and axonal growth following central and peripheral nervous system neuroinflammation. But its precise roles in these processes and involvement in spinal cord injury pathophysiology remain unclear. Western blot assay revealed that Ninjurin-1 levels in rats with spinal cord injury exhibited an upregulation until day 4 post-injury and slightly decreased thereafter compared with sham controls. Immunohistochemistry analysis revealed that Ninjurin-1 immunoreactivity in rats with spinal cord injury sharply increased on days 1 and 4 post-injury and slightly decreased on days 7 and 21 post-injury compared with sham controls. Ninjurin-1 immunostaining was weak in vascular endothelial cells, ependymal cells, and some glial cells in sham controls while it was relatively strong in macrophages, microglia, and reactive astrocytes. These findings suggest that a variety of cells, including vascular endothelial cells, macrophages, and microglia, secrete Ninjurin-1 and they participate in the pathophysiology of compression-induced spinal cord injury. All experimental procedures were approved by the Care and Use of Laboratory Animals of Jeju National University (approval No. 2018-0029) on July 6, 2018.

Key words: astrocytes, clip compression injury, macrophage, microglia, neuroinflammation, Ninjurin-1, rat, spinal cord