中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (8): 595-560.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage

  

  • 收稿日期:2011-10-07 修回日期:2012-01-06 出版日期:2012-03-15 发布日期:2012-03-15

Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage

Ruizhi Wang1, Dongning Hao2, Wei Shi1, Jingnan Pu1, Zizhang Wang3   

  1. 1 Department of Neurosurgery, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
    2 Department of Neurosurgery, Yulin First People’s Hospital, Yulin 718000, Shaanxi Province, China
    3 Department of Head and Neck Surgery, Tumor Hospital of Shaanxi Province, Xi’an 710061, Shaanxi Province, China
  • Received:2011-10-07 Revised:2012-01-06 Online:2012-03-15 Published:2012-03-15
  • Contact: Wei Shi, Professor, Doctoral supervisor, Department of Neurosurgery, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China wrz510@163.com
  • About author:Ruizhi Wang☆, M.D., Attending physician, Department of Neurosurgery, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China

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Abstract:

In the present study, a rat model of non-traumatic intracerebral hemorrhage was established by type IV collagenase injection into the right globus pallidus. Bax and Bcl-2 expression in tissues surrounding hematomas was significantly increased within 14 days after injury, and it then gradually decreased. Vascular endothelial growth factor, Flk-1 and Flt-1 mRNA expression gradually increased over time. After intraperitoneal injection with minocycline, Bax expression was decreased 1 day after intracerebral hemorrhage. Flk-1 and Flt-1 mRNA expression was decreased after minocycline injection, but Bcl-2 expression was increased, and vascular endothelial growth factor mRNA expression was decreased between 4-14 days. These results indicated that protective effects of minocycline on nerve tissues were associated with increased Bcl-2 expression and decreased Bax expression in the early stage after intracerebral hemorrhage. In the late stage, minocycline downregulated vascular endothelial growth factor and its receptor expression to inhibit brain tissue self-repair.

Key words: intracerebral hemorrhage, minocycline, vascular endothelial growth factor, apoptosis, inflammation, Bax, Bcl-2, Flk-1, Flt-1, neural regeneration