中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (7): 496-500.

• 原著:颅神经损伤修复保护与再生 • 上一篇    下一篇

Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy

  

  • 收稿日期:2011-12-22 修回日期:2012-02-06 出版日期:2012-03-05 发布日期:2012-03-05

Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy

Jing Chen1, Hu Hao2, Guoqing Guo3, Sitao Li2, Xin Xiao2   

  1. 1 Institute of Gastroenterology, Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong Province, China
    2 Laboratory of Inborn Errors of Metabolism, Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong Province, China
    3 Department of Anatomy, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China
  • Received:2011-12-22 Revised:2012-02-06 Online:2012-03-05 Published:2012-03-05
  • Contact: Hu Hao, Master, Attending physician, Laboratory of Inborn Errors of Metabolism, Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong Province, China freehaotiger@126.com
  • About author:Jing Chen★, Master, Assistant researcher, Institute of Gastroenterology, Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong Province, China

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Abstract:

Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significantly reduced. After treatment with the Rho kinase inhibitor Y27632, a significant increase in the numbers of these neurites was observed. Our experimental findings indicate that the Rho-kinase pathway is closely associated with the neurites of hippocampal neurons.

Key words: atomic force microscopy, Rho-kinase, nerve cells, neurites, hippocampus, rats, neural regeneration