中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (7): 506-510.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

Differentiation of fetal pancreatic stem cells into neuron-like and islet-like cells in vitro

  

  • 收稿日期:2011-11-18 修回日期:2012-02-17 出版日期:2012-03-05 发布日期:2012-03-05

Differentiation of fetal pancreatic stem cells into neuron-like and islet-like cells in vitro

Xiufeng Hua1, Yanwei Wang2, Peiwen Lian2, Shouxin Zhang2, Jianyuan Li2, Haiyan Wang2, Shulin Chen1, Wei Gao1   

  1. 1 Department of Endocrinology, Yuhuangding Hospital, Yantai 264000，Shandong Province, China
    2 Research Center of Stem Cell Engineering of Shandong, Central Laboratory of Yuhuangding Hospital, Yantai 264000, Shandong Province, China
  • Received:2011-11-18 Revised:2012-02-17 Online:2012-03-05 Published:2012-03-05
  • Contact: Xiufeng Hua, Department of Endocrinology, Yuhuangding Hospital, Yantai 264000, Shandong Province, China hxf680901@163.com
  • About author:Xiufeng Hua★, Master, Associate chief physician, Department of Endocrinology, Yuhuangding Hospital, Yantai 264000，Shandong Province, China

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Abstract:

Pancreatic stem cells were isolated and cultured from aborted human fetal pancreases of gestational age 14-20 weeks. They were seeded at a density of 1 × 104 in serum-free media for differentiation into neuron-like cells, expressing β-tubulin III and glial fibrillary acidic protein. These neuron-like cells displayed a synapse-like morphology and appeared to form a neuronal network. Pancreatic stem cells were also seeded at a density of 1 × 105 for differentiation into islet-like cells, expressing insulin and glucagon, with an islet-like morphology. These cells had glucose-stimulated secretion of human insulin and C-peptide. Results suggest that pancreatic stem cells can be differentiated into neuron-like and islet-like cells.

Key words: fetal pancreas, pancreatic stem cells, differentiation, islet-like cells, neuron-like cells, neural regeneration