中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (12): 2479-2485.doi: 10.4103/1673-5374.313067

• 原著:退行性病与再生 • 上一篇    下一篇

养老机构老年人多模式锻炼方案对其生活质量、表观遗传标记和脑源性神经营养因子的影响

  

  • 出版日期:2021-12-15 发布日期:2021-05-15

Effects of a multimodal exercise protocol on functional outcomes, epigenetic modulation and brain-derived neurotrophic factor levels in institutionalized older adults: a quasi-experimental pilot study

Iasmin Fraga1, Camila Weber2, Wériton Baldo Galiano2, Lucio Iraci2, Mariana Wohlgemuth2, Gabriela Morales2, Camila Cercato2, Juliana Rodriguez2, Daniela Pochmann3, Caroline Dani3, Pérsia Menz4, Adriane Dal Bosco2, Viviane Rostirola Elsner1, 2, 3, *   

  1. 1Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil; 2Curso de Fisioterapia do Centro Universitário Metodista-IPA, Porto Alegre, RS, Brasil; 3Programa de Pós-Graduação em Biociências e Reabilitação, Centro Universitário Metodista-IPA, Porto Alegre, RS, Brasil; 4Physiotherapist, working in Long-Term Institutions, Porto Alegre, RS, Brasil
  • Online:2021-12-15 Published:2021-05-15
  • Contact: Viviane Rostirola Elsner, elsner.viviane@gmail.com.

摘要:

有研究表明,体育锻炼对老年人的功能,认知和生活质量的好处,但尚不清楚其中涉及的分子机制。为了解多模式运动干预对养老机构老年人的功能结局、生活质量(QoL)、表观遗传标记和脑源性神经营养因子(BDNF)水平的影响,开展准实验性试验研究,纳入年龄为73.38±11.28岁,且无痴呆的老年人8名,主要为女性(87.5%)。给予老年人8周的多模式锻炼方案,每周2次,每次60分钟,该方案包括心肺功能、运动功能、认知功能训练。干预前后应用Berg量表、起立行走试验、简明精神状态量表、WHOQOL-BREF问卷评估老年人平衡、运动功能、认知功能和生活质量。同时采集血样进行总体组蛋白H3乙酰化水平和脑源性神经营养因子水平检测。干预后,老年人认知功能、平衡能力、运动功能、精神状态和生活质量均明显改善;且外周血总体组蛋白H3乙酰化水平有增加趋势,而脑源性神经营养因子水平保持不变。说明为期8周的多模式锻炼方案利于改善养老机构老年人的功能结局和生活质量,该作用似乎与组蛋白的高乙酰化状态有关。

https://orcid.org/0000-0003-4158-181X (Viviane Rostirola Elsner)

Abstract: Epigenetic changes have been shown to be associated with both aging process and aging-related diseases. There is evidence regarding the benefits of physical activity on the functionality, cognition, and quality of life of institutionalized older adults, however, the molecular mechanisms involved are not elucidated. The purpose of this pilot study was to investigate the effects of a multimodal exercise intervention on functional outcomes, cognitive performance, quality of life (QOL), epigenetic markers and brain-derived neurotrophic factor (BDNF) levels among institutionalized older adult individuals. Participants (n = 8) without dementia who were aged 73.38 ± 11.28 years and predominantly female (87.5%) were included in this quasi-experimental pilot study. A multimodal exercise protocol (cardiovascular capacity, strength, balance/agility and flexibility, perception and cognition) consisted of twice weekly sessions (60 minutes each) over 8 weeks. Balance (Berg Scale), mobility (Timed Up and Go test), functional capacity (Six-Minute Walk test), cognitive function (Mini-Mental State Examination) and QOL (the World Health Organization Quality of Life-BREF Scale questionnaire) were evaluated before and after the intervention. Blood sample (15 mL) was also collected before and after intervention for analysis of biomarkers global histone H3 acetylation and brain-derived neurotrophic factor levels. Significant improvements were observed in cognitive function, balance, mobility, functional capacity and QOL after the intervention. In addition, a tendency toward an increase in global histone H3 acetylation levels was observed, while brain-derived neurotrophic factor level remained unchanged. This study provided evidence that an 8-week multimodal exercise protocol has a significant effect on ameliorating functional outcomes and QOL in institutionalized older adult individuals. In addition, it was also able to promote cognitive improvement, which seems to be partially related to histone hyperacetylation status. The Ethics Research Committee of Centro Universitário Metodista-IPA, Brazil approved the current study on June 6, 2019 (approval No. 3.376.078). 

Key words: aging, balance, brain-derived neurotrophic factor, cognition, epigenetics, physical exercise, quality of life, risk of falling

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