中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (5): 332-340.

• 原著:退行性病与再生 • 上一篇    下一篇

Analysis of hippocampal gene expression profile of Alzheimer’s disease model rats using genome chip bioinformatics

  

  • 收稿日期:2011-08-27 修回日期:2011-12-20 出版日期:2012-02-15 发布日期:2012-02-15

Analysis of hippocampal gene expression profile of Alzheimer’s disease model rats using genome chip bioinformatics

Yinghong Li1, Zhengzhi Wu1, 2, Yu Jin1, Anmin Wu1, Meiqun Cao2, Kehuan Sun1, Xiuqin Jia1, Manyin Chen1   

  1. 1  First Affiliated Hospital of Shenzhen University/Second People’s Hospital of Shenzhen City, Shenzhen 518035, Guangdong Province, China
    2  Second Clinical Medical College of Jinan University/Shenzhen Institute of Geriatrics, Shenzhen 518020, Guangdong Province, China
  • Received:2011-08-27 Revised:2011-12-20 Online:2012-02-15 Published:2012-02-15
  • Contact: Zhengzhi Wu, Professor, Researcher, Doctoral supervisor, First Affiliated Hospital of Shenzhen University/Second People’s Hospital of Shenzhen City, Shenzhen 518035, Guangdong Province, China; Second Clinical Medical College of Jinan University/Shenzhen Institute of Geriatrics, Shenzhen 518020, Guangdong Province, China szwzz001@163.com
  • About author:Yinghong Li☆, Ph.D., Associate professor, First Affiliated Hospital of Shenzhen University/Second People’s Hospital of Shenzhen City, Shenzhen 518035, Guangdong Province, China

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Abstract:

In this study, an Alzheimer’s disease model was established in rats through stereotactic injection of condensed amyloid beta 1-40 into the bilateral hippocampus, and the changes of gene expression profile in the hippocampus of rat models and sham-operated rats were compared by genome expression profiling analysis. Results showed that the expression of 50 genes was significantly up-regulated (fold change ≥ 2), while 21 genes were significantly down-regulated in the hippocampus of Alzheimer’s disease model rats (fold change ≤ 0.5) compared with the sham-operation group. The differentially expressed genes are involved in many functions, such as brain nerve system development, neuronal differentiation and functional regulation, cellular growth, differentiation and apoptosis, synaptogenesis and plasticity, inflammatory and immune responses, ion channels/transporters, signal transduction, cell material/energy metabolism. Our findings indicate that several genes were abnormally expressed in the metabolic and signal transduction pathways in the hippocampus of amyloid beta 1-40-induced rat model of Alzheimer’s disease, thereby affecting the hippocampal and brain functions.

Key words: amyloid beta 1-40, Alzheimer’s disease, hippocampus, genome chip, gene expression profile, neural regeneration