中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (8): 1827-1832.doi: 10.4103/1673-5374.332153

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

胫神经横断后靶向肌肉再支配对大鼠脊髓运动神经元的影响

  

  • 出版日期:2022-08-15 发布日期:2022-01-22
  • 基金资助:
    国家自然科学基金(81760416,8196049,81927804);贵州省科技计划(黔科合基础(2017)1226);贵州省普通高等学校科技拔尖人才支持计划(黔教合KY字(2018)056)

Effects of targeted muscle reinnervation on spinal cord motor neurons in rats following tibial nerve transection

Wei Lu1, 2, Jian-Ping Li2, Zhen-Dong Jiang2, Lin Yang2, *, Xue-Zheng Liu1, *   

  1. 1Department of Human Anatomy, School of Basic Medical Scinences, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China; 2Department of Human Anatomy, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong Province, China
  • Online:2022-08-15 Published:2022-01-22
  • Contact: Xue-Zheng Liu, MD, 544758611@qq.com; Lin Yang, MD, iyzwll@aliyun.com.
  • Supported by:
    The study was funded by the National Natural Science Foundation of China, Nos. 81760416, 81960419, 81927804 (all to LY), Science and Technology foundation of Guizhou Province, No. [2017]1226 (to LY), and Support Plan for High Level Talents in Guizhou High Education Institutions, No. KY[2018]056 (to LY).

摘要:

靶向肌肉神经移植(靶向肌肉再支配)是一种外科干预手段,用于将截肢后残留的周围神经转移到目标肌肉,使目标肌肉成为功能重建的运动控制信息来源。上肢截肢后的靶向肌肉神经移植可以建立有效的肌肉和神经功能重建,但靶向肌肉神经移植对受伤运动神经元的影响仍不清楚。实验发现,后肢靶向肌肉神经移植后,胫神经横断小鼠后肢运动功能和肌肉萎缩得到了改善。靶向肌肉神经移植通过增加胫神经的轴突数量和髓鞘厚度,也增加了脊髓前角运动神经元和脊髓运动神经元标志物胆碱乙酰转移酶阳性细胞的数量及突触可塑性相关的突触素免疫荧光强度。结果表明,靶向肌肉再支配可以使胫神经残端与靶肌肉建立新的连接,恢复运动功能。

https://orcid.org/0000-0003-1372-8677 (Lin Yang)

Abstract: Targeted muscle reinnervation (TMR) is a surgical procedure used to transfer residual peripheral nerves from amputated limbs to targeted muscles, which allows the target muscles to become sources of motor control information for function reconstruction. However, the effect of TMR on injured motor neurons is still unclear. In this study, we aimed to explore the effect of hind limb TMR surgery on injured motor neurons in the spinal cord of rats after tibial nerve transection. We found that the reduction in hind limb motor function and atrophy in mice caused by tibial nerve transection improved after TMR. TMR enhanced nerve regeneration by increasing the number of axons and myelin sheath thickness in the tibial nerve, increasing the number of anterior horn motor neurons, and increasing the number of choline acetyltransferase-positive cells and immunofluorescence intensity of synaptophysin in rat spinal cord. Our findings suggest that TMR may enable the reconnection of residual nerve fibers to target muscles, thus restoring hind limb motor function on the injured side.

Key words: function reconstruction, motor neuron, nerve injury, nerve implant, Nissl staining, spinal cord, synaptophysin, targeted muscle reinnervation, tibial nerve, transection