中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (4): 273-277.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

N-methyl-D-aspartate receptor subtype 3A promotes apoptosis in developing mouse brain exposed to hyperoxia

  

  • 收稿日期:2011-09-24 修回日期:2011-12-22 出版日期:2012-02-05 发布日期:2012-02-05

N-methyl-D-aspartate receptor subtype 3A promotes apoptosis in developing mouse brain exposed to hyperoxia

Jimei Li1, 2, Shanping Yu3, Zhongyang Lu2, Osama Mohamad3, Ling Wei2, 3   

  1. 1 Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
    2 Departments of Pathology and Laboratory Medicine, Medical University of South Carolina, SC 29425, USA
    3 Department of Anesthesiology, Emory University School of Medicine, Atlanta, GA 30322, USA
  • Received:2011-09-24 Revised:2011-12-22 Online:2012-02-05 Published:2012-02-05
  • Contact: Ling Wei, M.D., Professor, Departments of Pathology and Laboratory Medicine, Medical University of South Carolina, SC 29425, USA; Department of Anesthesiology, Emory University School of Medicine, Atlanta, GA 30322, USA lwei7@emory.edu
  • About author:Jimei Li, Professor, Chief physician, Doctoral supervisor, Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Departments of Pathology and Laboratory Medicine, Medical University of South Carolina, SC 29425, USA

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Abstract:

In the present study, 7 day postnatal C57/BL6 wild-type mice (hyperoxia group) and 7 day postnatal N-methyl-D-aspartate receptor subtype 3A knockout mice (NR3A KO group) were exposed to 75% oxygen and 15% nitrogen in a closed container for 5 days. Wild-type mice raised in normoxia served as controls. TdT-mediated dUTP nick end labeling (TUNEL)/neuron-specific nuclear protein (NeuN) and 5-bromo-2’-deoxyuridine (BrdU)/NeuN immunofluorescence staining showed that the number of apoptotic cells and the number of proliferative cells in the dentate subgranular zone significantly increased in the hyperoxia group compared with the control group. However, in the same hyperoxia environment, the number of apoptotic cells and the number of proliferative cells significantly decreased in the NR3A KO group compared with hyperoxia group. TUNEL+/NeuN+ and BrdU+/NeuN+ cells were observed in the NR3A KO and the hyperoxia groups. These results demonstrated that the NR3A gene can promote cell apoptosis and mediate the potential damage in the developing brain induced by exposure to non-physiologically high concentrations of oxygen.

Key words: N-methyl-D-aspartate receptor subtype 3A, apoptosis, cell proliferation, hyperoxia, developing brain, nerve cells, mouse, neurobiology, neural regeneration