中国神经再生研究(英文版)

中国神经再生研究(英文版)

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

DNA degradation within mouse brain and dental pulp cells 72 hours postmortem

  

  • 收稿日期:2011-09-24 修回日期:2011-11-22 出版日期:2012-02-05 发布日期:2012-02-05

DNA degradation within mouse brain and dental pulp cells 72 hours postmortem

Jilong Zheng1, 2, Xiaona Li3, Di Shan4, Han Zhang5, Dawei Guan1   

  1. 1 School of Forensic Medicine, China Medical University, Shenyang 110001, Liaoning Province, China
    2 School of Forensic Medicine, China Criminal Police University, Shenyang 110035, Liaoning Province, China
    3 Department of Chemistry, China Medical University School of Basic Medicine, Shenyang 110001, Liaoning Province, China
    4 North Railway Police Station, Shenyang Public Security Bureau, Shenyang 110013, Liaoning Province, China
    5 Department of Police Dog Technology, China Criminal Police University, Shenyang 110035, Liaoning Province, China
  • Received:2011-09-24 Revised:2011-11-22 Online:2012-02-05 Published:2012-02-05
  • Contact: Dawei Guan, Doctor, Professor, School of Forensic Medicine, China Medical University, Shenyang 110001, Liaoning Province, China dwguan@mail.cmu.edu.cn
  • About author:Dawei Guan, Doctor, Professor, School of Forensic Medicine, China Medical University, Shenyang 110001, Liaoning Province, China dwguan@mail.cmu.edu.cn

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Abstract:

In this study, we sought to elucidate the process of DNA degradation in brain and dental pulp cells of mice, within postmortem 0-72 hours, by using the single cell gel electrophoresis assay and professional comet image analysis and processing techniques. The frequency of comet-like cells, the percentage of tail DNA, tail length, tail moment, Olive moment and tail area increased in tandem with increasing postmortem interval. In contrast, the head radius, the percentage of head DNA and head area showed a decreasing trend. Linear regression analysis revealed a high correlation between these parameters and the postmortem interval. The findings suggest that the single cell gel electrophoresis assay is a quick and sensitive method to detect DNA degradation in brain and dental pulp cells, providing an objective and accurate new way to estimate postmortem interval.

Key words: DNA degradation, single cell gel electrophoresis, postmortem interval, brain, dental pulp