中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (12): 2730-2736.doi: 10.4103/1673-5374.339497

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

硬膜外振荡场刺激提高脊髓损伤后轴突再生能力和髓鞘化程度

  

  • 出版日期:2022-12-15 发布日期:2022-05-05
  • 基金资助:
    斯洛伐克科学院科学资助机构的资助(2/0098/20;2/0145/21); 斯洛伐克研发署(APVV19-0324;APVV-18-0163);欧洲区域发展基金共同资助IMTS:313011V344

Epidural oscillating field stimulation increases axonal regenerative capacity and myelination after spinal cord trauma

Maria Bacova, Katarina Bimbova, Alexandra Kisucka, Nadezda Lukacova, Jan Galik*   

  1. Institute of Neurobiology of Biomedical Research Center, Slovak Academy of Sciences, Soltesovej, Kosice, Slovak Republic
  • Online:2022-12-15 Published:2022-05-05
  • Contact: Jan Galik, PhD, galik@saske.sk.
  • Supported by:
    This study was supported by grants of the Scientific Grant Agency of the Slovak Academy of Sciences: 2/0098/20 (JG); 2/0145/21 (NL); the Slovak Research and Development Agency: APVV19-0324 (to NL); APVV-18-0163 (MB); the Operational Programme Integrated Infrastructure for the project: Long-term strategic research of prevention, intervention and mechanisms of obesity and its comorbidities, IMTS: 313011V344, co-financed by the European Regional Development Fund” (JG)

摘要:

定期改变电流极性的振荡场刺激(OFS)可以刺激、支持和潜在地引导脊髓损伤(SCI)后的感觉和运动神经纤维的生长。作者既往实验证明了振荡场刺激对生存4周脊髓损伤动物可产生有益作用。为进一步研究伤后立即行长期的50µA振荡场刺激对生存8周的脊髓损伤大鼠髓鞘再生、轴突和少突胶质细胞存活以及脊髓功能恢复的影响。脊柱组织的组织病理学分析表明,硬膜外振荡场刺激对减少病变部位附近节段的脊髓损伤后的组织和髓鞘损失有很大影响。脊髓组织受影响最严重的区域的定量荧光分析显示,脊髓损伤大鼠在接受振荡场刺激干预后,幸存的轴突和少突胶质细胞更多。脊髓组织中神经丝蛋白(NF-l)、新生的轴突标志物生长相关蛋白和髓鞘碱性蛋白的蛋白水平明显增加,表明振荡场刺激在脊髓损伤后轴突和髓鞘再生中起支持作用。此外,振荡场刺激干预后脊髓损伤大鼠的感觉和运动功能明显改善。这些数据表明,脊髓损伤后立即行长期振荡场刺激可以在损伤的急性期触发神经再生过程,为受损脊髓组织的恢复提供一个较好的微环境。

https://orcid.org/0000-0001-9581-9585 (Jan Galik)

Abstract: Oscillating field stimulation (OFS) with regular alterations in the polarity of electric current is a unique, experimental approach to stimulate, support, and potentially guide the outgrowth of both sensory and motor nerve fibers after spinal cord injury (SCI). In previous experiments, we demonstrated the beneficial effects of OFS in a 4-week survival period after SCI. In this study, we observed the major behavioral, morphological, and protein changes in rats after 15 minutes of T9 spinal compression with a 40 g force, followed by long-lasting OFS (50 µA), over a 8-week survival period. Three groups of rats were analyzed: rats after T9 spinal compression (SCI group); SCI rats subjected to implantation of active oscillating field stimulator (OFS + SCI group); and SCI rats subjected to nonfunctional OFS (nOFS + SCI group). Histopathological analysis of spinal tissue indicated a strong impact of epidural OFS on the reduction of tissue and myelin loss after SCI in the segments adjacent to the lesion site. Quantitative fluorescent analysis of the most affected areas of spinal cord tissue revealed a higher number of spared axons and oligodendrocytes of rats in the OFS + SCI group, compared with rats in the SCI and nOFS + SCI groups. The protein levels of neurofilaments (NF-l), growth-associated protein-43 (marker for newly sprouted axons), and myelin basic protein in rats were signifiantly increased in the OFS + SCI group than in the nOFS + SCI and SCI groups. This suggests a supporting role of the OFS in axonal and myelin regeneration after SCI. Moreover, rats in the OFS + SCI group showed great improvements in sensory and motor functions than did rats in the nOFS + SCI and SCI groups. All these findings suggest that long-lasting OFS applied immediately after SCI can provide a good microenviroment for recovery of damaged spinal tissue by triggering regenreative processes in the acute phase of injury.

Key words: axonal regenerative capacity, behavioral assessment, epidural stimulation, motor recovery, myelin regeneration, oscillating field stimulation, spinal cord injury